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利用具有甲烷单加氧酶的全细胞甲烷氧化菌进行乙烷到乙醇的生物转化的开发和优化。

Development and Optimization of the Biological Conversion of Ethane to Ethanol Using Whole-Cell Methanotrophs Possessing Methane Monooxygenase.

机构信息

Department of Chemical Engineering, Kyung Hee University, Gyeonggi-do 17104, Korea.

Department of Chemical Engineering, Changwon University, Changwon 51140, Korea.

出版信息

Molecules. 2019 Feb 7;24(3):591. doi: 10.3390/molecules24030591.

DOI:10.3390/molecules24030591
PMID:30736408
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6384962/
Abstract

The biological production of ethanol from ethane for the utilization of ethane in natural gas was investigated under ambient conditions using whole-cell methanotrophs possessing methane monooxygenase. Several independent variables including ethane concentration and biocatalyst amounts, among other factors, were optimized for the enhancement of ethane-to-ethanol bioconversion. We obtained 0.4 g/L/h of volumetric productivity and 0.52 g/L of maximum titer in optimum batch reaction conditions. In this study, we demonstrate that the biological gas-to-liquid conversion of ethane to ethanol has potent technical feasibility as a new application of ethane gas.

摘要

利用具有甲烷单加氧酶的完整甲烷菌,在环境条件下从乙烷中生物生产乙醇,以利用天然气中的乙烷。研究了包括乙烷浓度和生物催化剂用量在内的多个独立变量,以优化提高乙烷到乙醇的生物转化。在最佳分批反应条件下,我们获得了 0.4 g/L/h 的比产率和 0.52 g/L 的最高浓度。在这项研究中,我们证明了将乙烷生物转化为乙醇的气体到液体的转化作为乙烷气体的新应用具有很强的技术可行性。

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Potential of Immobilized Whole-Cell Methylocella tundrae as a Biocatalyst for Methanol Production from Methane.固定化全细胞苔原甲基孢囊菌作为从甲烷生产甲醇的生物催化剂的潜力。
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