[Bsu50441——核酸内切限制酶AsuI的同裂酶]
[Bsu50441--isoschizomer of endonuclease restriction AsuI].
作者信息
Reva O M, Luk'ianchuk V V, Polishchuk L V
机构信息
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154, Zabolotny St., Kyiv, 03143, Ukraine.
出版信息
Mikrobiol Z. 2002 Jan-Feb;64(1):57-9.
Endonuclease of restriction of type II has been found in Bacillus subtilis B-5044 during testing of endophytic strains of cotton plants bacilli. The restrictase Bsu5044I hydrolysed DNA of M13mp18 phage in 4 sites; pUC19 DNA in 5 sites; pBR322 in 15 sites. There were a lot of sites restriction in DNAs of phages T7 and lambda. A comparison of electrophoretical divisions of Bsu5044I and Cfr13I fragments of phages and plasmid DNAs showed their identity. Thus, the found restrictase Bsu5044I is an isoschisomer of AsuI.
在对棉花植株芽孢杆菌内生菌株进行检测时，在枯草芽孢杆菌B - 5044中发现了II型限制性核酸内切酶。限制性内切酶Bsu5044I在4个位点水解M13mp18噬菌体的DNA；在5个位点水解pUC19 DNA；在15个位点水解pBR322。噬菌体T7和λ的DNA中有许多限制性位点。对噬菌体和质粒DNA的Bsu5044I和Cfr13I片段的电泳图谱进行比较，结果显示它们具有同一性。因此，所发现的限制性内切酶Bsu5044I是AsuI的同裂酶。
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