Sobczak-Filipiak M, Malicka E
Division of Pathological Anatomy, Department of Pathology, Faculty of Veterinary Medicine, Warsaw Agricultural University (SGGW), 272 Grochowska Street, 03-849 Warszawa, Poland.
Pol J Vet Sci. 2002;5(1):1-5.
The aim of the present study was to establish the relationship between estrogen receptor expression in neoplastic parenchymal cells and the type of canine mammary gland tumour. The research material included mammary gland tumours obtained from 66 dogs. Paraffin sections were stained with hematoxylin-eosin and immunohistochemically with monoclonal antibodies and LSAB/Peroxidase/Universal Kit. The estrogen receptor expression was observed in nuclei of neoplastic cells in about 59% of the cases and in the cytoplasm in about 89% of the cases. In about 20% of the cases the expression in the nuclei and in the cytoplasm was extremely weak. No correlation was found between the expression of estrogen receptors and the value of mitotic indexes in the neoplasms investigated.
本研究的目的是确定肿瘤实质细胞中雌激素受体表达与犬乳腺肿瘤类型之间的关系。研究材料包括从66只犬身上获取的乳腺肿瘤。石蜡切片用苏木精-伊红染色，并使用单克隆抗体和LSAB/过氧化物酶/通用试剂盒进行免疫组织化学染色。在约59%的病例中，在肿瘤细胞核中观察到雌激素受体表达，在约89%的病例中在细胞质中观察到表达。在约20%的病例中，细胞核和细胞质中的表达极其微弱。在所研究的肿瘤中，未发现雌激素受体表达与有丝分裂指数值之间存在相关性。
