Prasongwatana V, Tavichakorntrakool R, Sriboonlue P, Wongkham C, Bovornpadungkitti S, Premgamone A, Reungjuiy S
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Thailand.
Southeast Asian J Trop Med Public Health. 2001 Sep;32(3):648-53.
Samples of external oblique muscles were surgically removed from 45 renal stone patients and analyzed for their K, Na and Mg content. The muscle samples were also measured for membrane Na, K-ATPase activity from the assay of its K+-dependent 3-0-methyl fluorescein phosphatase (K+-dependent 3-0-MFPase) activity. The results showed that the mean muscle contents +/- SEM of K, Na and Mg were 65.2 +/- 1.7 (range, 41.1 to 86.1), 45.5 +/- 2.0 (range, 23.5 to 73.2) and 6.3 +/- 1.0 (range, 4.1 to 8.5) micromol/g wet weight, respectively. The mean activity +/- SEM of the K+-dependent 3-0-MFPase or the Na, K-ATPase was calculated by subtracting the activity of the basal-form from that of the total-3-0-MFPase, which was 113 +/- 21 (range, 11 to 177) nmol/g wet weight/minute. The activity of the Na, K-ATPase showed a significant correlation with muscle K-content (r = 0.52, p<0.001) and Mg content (r = 0.45, p<0.002). Though the external oblique muscles of renal stone patients in our study, as compared to data from other sources, had a considerably low concentration of K and Mg, they exhibited a good correlation with membrane-Na, K-ATPase activity. Our results, therefore, support previous observations made by other investigators.
从45例肾结石患者身上手术切除腹外斜肌样本，并分析其钾、钠和镁含量。还通过测定其依赖钾的3 - O - 甲基荧光素磷酸酶（K⁺依赖的3 - O - MFPase）活性来测量肌肉样本的膜钠钾ATP酶活性。结果显示，钾、钠和镁的平均肌肉含量±标准误分别为65.2±1.7（范围为41.1至86.1）、45.5±2.0（范围为23.5至73.2）和6.3±1.0（范围为4.1至8.5）微摩尔/克湿重。依赖钾的3 - O - MFPase或钠钾ATP酶的平均活性±标准误是通过从总3 - O - MFPase的活性中减去基础形式的活性来计算的，为113±21（范围为11至177）纳摩尔/克湿重/分钟。钠钾ATP酶的活性与肌肉钾含量（r = 0.52，p<0.001）和镁含量（r = 0.45，p<0.002）呈显著相关。尽管我们研究中肾结石患者的腹外斜肌与其他来源的数据相比，钾和镁的浓度相当低，但它们与膜钠钾ATP酶活性表现出良好的相关性。因此，我们的结果支持了其他研究者先前的观察结果。
