Waite E R, March J B
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, Midlothian, EH26 0PZ, UK
J Comp Pathol. 2002 Feb-Apr;126(2-3):171-82. doi: 10.1053/jcpa.2001.0540.
The immunogenicity of Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC) vaccines was investigated in BALB/c mice. Groups of mice were vaccinated with either (1) unconjugated capsular polysaccharide (CPS), (2) CPS covalently conjugated to ovalbumin via a carbodiimide reaction, (3) CPS non-covalently bound to latex microspheres, (4) CPS non-covalently complexed with rabbit anti-CPS IgG, and (5) whole inactivated, ultrasonically disrupted (WID) MmmSC. Only mice immunized with the CPS-ovalbumin conjugate exhibited a significant (P<0 small middle dot001) antibody response against CPS. Mice immunized with WID vaccine exhibited a high ELISA antibody titre against non-CPS (protein) antigens only. Mice given WID vaccine were immune against challenge with live MmmSC, and exhibited a significantly reduced degree of mycoplasmaemia (both in incidence and duration) as compared with non-vaccinated controls (P<0 small middle dot001). Mice immunized with the CPS-ovalbumin conjugate did not exhibit a reduction in mycoplasmaemia. The bactericidal activity of rabbit MmmSC-antiserum in an in-vitro growth inhibition test was related to the CPS antibody titre. This was not observed with antisera from the vaccinated mice. None of the mouse antisera exhibited growth inhibiting activity, irrespective of a high CPS or protein antibody titre (CPS-ovalbumin or WID vaccine groups, respectively). Thus, it would seem that protection against an MmmSC-induced mycoplasmaemia in the mouse is based upon cell-mediated rather than humoral immunity. The results suggest that conjugation to ovalbumin significantly increases the antibody response to CPS in the mouse; the lack of bactericidal activity of mouse anti-CPS as compared with rabbit anti-CPS in vitro suggests either that the titre of growth inhibiting antibodies is lower in the mouse or that the mechanism of growth inhibition differs between antibodies of the two species.
对丝状支原体丝状亚种小菌落生物型（MmmSC）疫苗在BALB/c小鼠中的免疫原性进行了研究。将小鼠分组，分别用以下物质进行免疫：（1）未结合的荚膜多糖（CPS）；（2）通过碳二亚胺反应与卵清蛋白共价结合的CPS；（3）与乳胶微球非共价结合的CPS；（4）与兔抗CPS IgG非共价复合的CPS；（5）完全灭活、超声破碎的（WID）MmmSC。只有用CPS-卵清蛋白偶联物免疫的小鼠对CPS表现出显著（P<0.001）的抗体反应。用WID疫苗免疫的小鼠仅对非CPS（蛋白质）抗原表现出高ELISA抗体滴度。接种WID疫苗的小鼠对活MmmSC攻击具有免疫力，与未接种疫苗的对照组相比，支原体血症的程度（发病率和持续时间）显著降低（P<0.001）。用CPS-卵清蛋白偶联物免疫的小鼠支原体血症没有减轻。在体外生长抑制试验中，兔MmmSC抗血清的杀菌活性与CPS抗体滴度相关。接种疫苗小鼠的抗血清未观察到这种情况。无论CPS或蛋白质抗体滴度高低（分别为CPS-卵清蛋白或WID疫苗组），小鼠抗血清均未表现出生长抑制活性。因此，似乎小鼠中针对MmmSC诱导的支原体血症的保护作用基于细胞介导免疫而非体液免疫。结果表明，与卵清蛋白偶联可显著增强小鼠对CPS的抗体反应；与兔抗CPS相比，小鼠抗CPS在体外缺乏杀菌活性，这表明要么小鼠中生长抑制抗体的滴度较低，要么两种物种抗体的生长抑制机制不同。
