Mwirigi Martin, Nkando Isabel, Olum Moses, Attah-Poku Samuel, Ochanda Horace, Berberov Emil, Potter Andrew, Gerdts Volker, Perez-Casal Jose, Wesonga Hezron, Soi Reuben, Naessens Jan
Kenya Agricultural and Livestock Research Organization, Biotechnology Research Institute, P.O. Box 14733, 00800, Nairobi, Kenya.
Kenya Agricultural and Livestock Research Organisation, Veterinary Science Research Institute, P.O. Box 32, 00902, Kikuyu, Kenya.
Vet Immunol Immunopathol. 2016 Oct 1;178:64-9. doi: 10.1016/j.vetimm.2016.07.002. Epub 2016 Jul 4.
Contagious Bovine Pleuropneumonia (CBPP) is a severe respiratory disease caused by Mycoplasma mycoides subsp. mycoides (Mmm) which is widespread in Africa. The capsule polysaccharide (CPS) of Mmm is one of the few identified virulence determinants. In a previous study, immunization of mice against CPS generated antibodies, but they were not able to prevent multiplication of Mmm in this model animal. However, mice cannot be considered as a suitable animal model, as Mmm does not induce pathology in this species. Our aim was to induce antibody responses to CPS in cattle, and challenge them when they had specific CPS antibody titres similar or higher than those from cattle vaccinated with the live vaccine. The CPS was linked to the carrier protein ovalbumin via a carbodiimide-mediated condensation with 1-ethyl-3(3-imethylaminopropyl) carbodiimide (EDC). Ten animals were immunized twice and challenged three weeks after the booster inoculation, and compared to a group of challenged non-immunized cattle. When administered subcutaneously to adult cattle, the vaccine elicited CPS-specific antibody responses with the same or a higher titre than animals vaccinated with the live vaccine. Pathology in the group of immunized animals was significantly reduced (57%) after challenge with Mmm strain Afadé compared to the non-immunized group, a figure in the range of the protection provided by the live vaccine.
牛传染性胸膜肺炎（CBPP）是一种由丝状支原体丝状亚种（Mmm）引起的严重呼吸道疾病，在非洲广泛传播。Mmm的荚膜多糖（CPS）是少数已确定的毒力决定因素之一。在先前的一项研究中，用CPS免疫小鼠可产生抗体，但这些抗体无法阻止Mmm在这种模式动物中的增殖。然而，由于Mmm不会在该物种中诱发病变，因此小鼠不能被视为合适的动物模型。我们的目标是在牛体内诱导针对CPS的抗体反应，并在它们的特异性CPS抗体滴度与接种活疫苗的牛相似或更高时对其进行攻毒。通过碳二亚胺介导的缩合反应，使用1-乙基-3（3-二甲基氨基丙基）碳二亚胺（EDC）将CPS与载体蛋白卵清蛋白连接。十只动物进行了两次免疫，并在加强接种三周后进行攻毒，并与一组攻毒的未免疫牛进行比较。当皮下注射给成年牛时，该疫苗引发的CPS特异性抗体反应的滴度与接种活疫苗的动物相同或更高。与未免疫组相比，用Mmm菌株阿法德攻毒后，免疫动物组的病变明显减少（57%），这一数值在活疫苗提供的保护范围内。
