Boren H G, Wright E C, Harris C C
J Histochem Cytochem. 1975 Dec;23(12):901-9. doi: 10.1177/23.12.1194673.
Emulsion sensitivity, latent image fading, and the effects of temperature, humidity, radiation dose and chemography on them were measured for NTB2 autoradiographic emulsion using quantitative methods. Sensitivity of NTB2 emulsion increased as the temperature during exposure increased, with the greatest increase per degree occurring between -20 degrees C. At 4 degrees C, emulsion sensitivity remained constant with time and radiation dose. Direct measurement of latent image fading showed no latent image fading for 60 weeks on slides exposed at 4 degrees C with Drierite. Slides exposed at 27 degrees C showed significant latent image fading and great variation between samples. High humidity decreased emulsion sensitivity and increased latent image fading. No evidence of either positive or negative chemography was found. The practical use of autoradiography requires an internal standard on each slide to correct for fluctuations in temperature and humidity during exposure time.
采用定量方法测量了NTB2放射自显影乳剂的乳剂敏感性、潜影消退以及温度、湿度、辐射剂量和化学显影对它们的影响。NTB2乳剂的敏感性随着曝光期间温度的升高而增加，每摄氏度的最大增幅出现在-20℃之间。在4℃时，乳剂敏感性随时间和辐射剂量保持恒定。潜影消退的直接测量表明，在4℃下用干燥剂曝光的载玻片上，60周内没有潜影消退。在27℃下曝光的载玻片显示出明显的潜影消退，且样品之间差异很大。高湿度降低了乳剂敏感性并增加了潜影消退。未发现正化学显影或负化学显影的证据。放射自显影的实际应用需要在每张载玻片上设置一个内标，以校正曝光期间温度和湿度的波动。
