Neely J E, Combs J W
J Histochem Cytochem. 1976 Oct;24(10):1057-64. doi: 10.1177/24.10.977937.
Five commonly used developers (Acufine, Rodinal, Dektol, D19 and Microdol X) and two popular nuclear gel emulsions (Kodak NTB-2 and Ilford L4) were tested in combinations for adequacy of development characteristics and the distribution of silver particle (grain) size measured by means of a reflectance microphotometer. All developer-emulsion combinations had some range of development time during which maximum development was accomplished with no occurrence of background grains, with the exceptions of the combinations of NTB2-Acufine and NTB2-D19. Ilford L4 emulsion obtained the narrowest grain size distribution with Rodinal and Acufine, followed by Dektol, and D19. The narrowest size distribution for Kodak NTB2 emulsion was achieved with D19 followed by Acufine, Dektol and Rodinal. Microdol-X had undesirable effects on the integrity of the individual grains of both emulsions. The criteria are discussed for selecting the most advantageous emulsion-developer system for the particular mode of evaluation (visual, photometric, television camera) to be applied to the finished autoradiographic specimens.
对五种常用显影剂（Acufine、Rodinal、Dektol、D19和Microdol X）和两种常用的核乳胶（柯达NTB - 2和伊尔福L4）进行了组合测试，以评估显影特性是否充分，以及通过反射式显微光度计测量银颗粒（晶粒）大小的分布情况。除了NTB2 - Acufine和NTB2 - D19的组合外，所有显影剂 - 乳胶组合都有一定的显影时间范围，在此范围内可实现最大显影且无背景颗粒出现。伊尔福L4乳胶与Rodinal和Acufine组合时获得的晶粒尺寸分布最窄，其次是Dektol和D19。柯达NTB2乳胶与D19组合时获得的尺寸分布最窄，其次是Acufine、Dektol和Rodinal。Microdol - X对两种乳胶的单个晶粒完整性有不良影响。讨论了为应用于最终放射自显影片的特定评估模式（视觉、光度测量、电视摄像）选择最有利的乳胶 - 显影剂系统的标准。
