Lupulescu A P, Birmingham D J
J Invest Dermatol. 1975 Nov;65(5):419-22. doi: 10.1111/1523-1747.ep12607986.
The effect of acetone and kerosene on the synthesis of protein, DNA, and collagen was studied by electron microscopic autoradiography using [3H]leucine, [3H]thymidine, and [3H]proline as tracers in human skin. Quantitative analyses following concomitant administration of tritiated leucine and acetone or kerosene demonstrated, at 90 min, a marked decrease in silver grains as compared to control or nonexposed areas. Incorporation of tritiated thymidine is moderately stimulated only by acetone, whereas radioactive proline distribution is not significantly affected. Electron microscopic autoradiograms revealed that tritiated leucine is distributed over all epidermal cells, mostly in the stratum spinosum of control epidermis; a marked decrease of silver grains from [3H]leucine followed both lipid solvent exposures. The autoradiographic reaction is specifically located over cytoplasmic organelles, such as polysomes, endoplasmic reticulum, and especially tonofilaments. Tritiated thymidine resulted in silver grains mostly over nuclear chromatin and these were moderatly increased after acetone application, whereas the incorporation of radioactive proline in the fibroblasts and collagen fibrils were not significantly influenced. These investigations indicate a dissociated effect of lipid solvents on protein, DNA, and collagen synthesis in human skin.
在人体皮肤中，以[³H]亮氨酸、[³H]胸腺嘧啶核苷和[³H]脯氨酸作为示踪剂，采用电子显微镜放射自显影术研究了丙酮和煤油对蛋白质、DNA和胶原蛋白合成的影响。在同时给予氚标记的亮氨酸与丙酮或煤油后进行的定量分析表明，在90分钟时，与对照或未暴露区域相比，银颗粒显著减少。仅丙酮适度刺激氚标记胸腺嘧啶核苷的掺入，而放射性脯氨酸的分布未受到显著影响。电子显微镜放射自显影片显示，氚标记的亮氨酸分布于所有表皮细胞，在对照表皮的棘层中分布最多；两种脂质溶剂暴露后，[³H]亮氨酸的银颗粒均显著减少。放射自显影反应特异性地位于细胞质细胞器上，如多核糖体、内质网，尤其是张力丝。氚标记的胸腺嘧啶核苷产生的银颗粒大多位于核染色质上，丙酮处理后这些银颗粒适度增加，而放射性脯氨酸在成纤维细胞和胶原纤维中的掺入未受到显著影响。这些研究表明脂质溶剂对人体皮肤中蛋白质、DNA和胶原蛋白合成具有解离效应。
