Reidenberg M M, Caccese R W
J Lab Clin Med. 1975 Dec;86(6):997-1002.
The problems of confirming that a suspected drug allergy exists and which of the administered drugs is the allergen were studied in 43 patients in whom such a diagnosis was considered. Heparinized whole blood was drawn from each patient and 1 ml. aliquots of the supernatant leucocyte-rich plasma were cultured for 3 to 5 days. Individual drugs were added to cultures at the start of incubation. Thymidine3H was added and the incorporated radioactivity measured. Control cultures were done concurrently. Twelve patients had increased thymidine uptake by their drug-treated cells (positive lymphocyte transformation tests). Five patients with positive tests had them repeated. Thymidine uptake decreased with time after the suspect drug was stopped and all the tests eventually became negative. The lymphocyte transformation test appears to be clinically useful for some patients and can confirm suspected drug allergy at times. Its further development appears warranted.
对43例被认为存在此类诊断的患者，研究了确认疑似药物过敏是否存在以及所使用的药物中哪一种是过敏原的问题。从每位患者采集肝素化全血，并将1毫升富含白细胞的血浆上清液等份培养3至5天。在培养开始时将个别药物加入培养物中。加入3H-胸腺嘧啶核苷并测量掺入的放射性。同时进行对照培养。12例患者经药物处理的细胞摄取胸腺嘧啶核苷增加（淋巴细胞转化试验阳性）。5例试验阳性的患者重复进行了试验。停用可疑药物后，胸腺嘧啶核苷摄取随时间下降，所有试验最终均变为阴性。淋巴细胞转化试验对某些患者似乎具有临床实用性，有时可以确认疑似药物过敏。其进一步发展似乎是有必要的。
