Domej Wolfgang, Schwaberger Günther, Tilz Gernot Peter, Földes-Papp Zeno, Demel Ulrike, Lang Johanna, von Duvillard Serge Petelin
Department of Internal Medicine, Graz University M. S. and Hospital, Graz, Austria.
Chest. 2002 Apr;121(4):1111-6. doi: 10.1378/chest.121.4.1111.
Eosinophils contain granule proteins such as eosinophil cationic protein (ECP) that have proinflammatory effects on airways. ECP may be released on activation of eosinophils into the plasma and is widely used as a marker of bronchial hyperreactivity and allergic inflammation. Environmental factors as well as intense physical exertion may influence eosinophil-related bronchial hyperreactivity.
To investigate the effect of endurance exercise at moderate altitude on levels of circulating eosinophils, serum ECP, serum osmolality (sOS), and dynamic pulmonary function parameters in healthy mountaineers.
Alpine field study performed in the Alps of Upper Styria in Austria. Type of exercise: Ascent of a mountain at maximal speed.
Thirty healthy male volunteers from a troop of military mountaineers.
Mean ECP concentration increased by 66% at the summit checkpoint (H2) and remained at 63% above baseline (base checkpoint [H0]) after descent (H4), while the blood eosinophil count decreased concomitantly from 250/microL at H0 (preexercise) to 118/microL (53%) at H2 and to 22/microL (81%) at H4. The total serum ECP concentration adjusted to sOS correlated negatively with blood eosinophil count (r = - 0.37; p < 0.0001) and PaO(2) (r = - 0.34; p < 0.001), but positively with the peak expiratory flow (PEF) [r = 0.45; p < 0.0001]. Although sOS correlated with serum ECP at H2 (r = 0.47; p = 0.02) and at 12 h after the start of the experiment (H12) [r = 0.57; p = 0.003], the relationship between total ECP and sOS (r = 0.19; p = 0.034) was less pronounced. FEV(1) in percentage of FVC (%FEV(1)/FVC) [the Tiffenau test], forced expiratory flow rate at 25% of vital capacity, and PEF were significantly higher at H2 than at H0 and H4. %FEV(1)/FVC decreased to 88% (p < 0.01) and 83% (p < 0.001) predicted at H12 and 24 h after start of the experiment, respectively.
Results provide strong evidence for nonspecific activation of blood eosinophils during prolonged intense aerobic exercise at moderate altitude, modifying both eosinophil dynamics and regulation of ECP release in healthy subjects.
嗜酸性粒细胞含有诸如嗜酸性粒细胞阳离子蛋白（ECP）等颗粒蛋白，这些蛋白对气道具有促炎作用。ECP可在嗜酸性粒细胞激活后释放到血浆中，并被广泛用作支气管高反应性和过敏性炎症的标志物。环境因素以及剧烈的体力活动可能会影响与嗜酸性粒细胞相关的支气管高反应性。
探讨中等海拔耐力运动对健康登山者循环嗜酸性粒细胞水平、血清ECP、血清渗透压（sOS）及动态肺功能参数的影响。
在奥地利上施蒂里亚州阿尔卑斯山进行的高山实地研究。运动类型：以最快速度登山。
来自一支军事登山队的30名健康男性志愿者。
在山顶检查点（H2）时，平均ECP浓度增加了66%，下山后（H4）仍比基线（基础检查点 [H0]）高出63%，而血液嗜酸性粒细胞计数则相应地从H0（运动前）时的250/μL降至H2时的118/μL（53%），并在H4时降至22/μL（81%）。经sOS调整后的血清总ECP浓度与血液嗜酸性粒细胞计数（r = -0.37；p < 0.0001）及动脉血氧分压（PaO₂）（r = -0.34；p < 0.001）呈负相关，但与呼气峰值流速（PEF）呈正相关[r = 0.45；p < 0.0001]。尽管sOS在H2时（r = 0.47；p = 0.02）及实验开始后12小时（H12）时[r = 0.57；p = 0.003]与血清ECP相关，但总ECP与sOS之间的关系（r = 0.19；p = 0.034）并不那么显著。H2时，一秒用力呼气容积占用力肺活量的百分比（%FEV₁/FVC）[蒂费瑙试验]、肺活量25%时的用力呼气流量及PEF均显著高于H0和H4时。实验开始后12小时及24小时时，%FEV₁/FVC分别降至预测值的88%（p < 0.01）和83%（p < 0.001）。
结果提供了有力证据，表明在中等海拔长时间剧烈有氧运动期间，血液嗜酸性粒细胞会发生非特异性激活，从而改变健康受试者的嗜酸性粒细胞动态变化及ECP释放的调节。
