从嗜钙裂须藻（Ag.）Gomont（蓝细菌）中分离得到的脂多糖的组成和生物学特性。
Composition and biological properties of lipopolysaccharides isolated from Schizothrix calcicola (Ag.) Gomont (Cyanobacteria).
作者信息
Keleti G, Sykora J L, Lippy E C, Shapiro M A
出版信息
Appl Environ Microbiol. 1979 Sep;38(3):471-7. doi: 10.1128/aem.38.3.471-477.1979.
Abstract
The most common cyanobacterium contaminating drinking water systems in southwestern Pennsylvania is Schizothrix calcicola. Lipoplysaccharides (LPS) were isolated from this species by hot phenol-water extraction. The polysaccharide moiety was composed of glucosamine, galactose, glucose, mannose, xylose and rhamnose. The lipid A part contained beta-hydroxylauric, myristic, pentadecanoic, palmitic, beta-hydroxypalmitic, stearic, oleic, and linoleic acids. In contrast to many LPS isolated from Enterobacteriaceae, the dominant component was not beta-hydroxymyristic but beta-hydroxypalmitic acid. The LPS induced Limulus lysate gelation and Schwartzman reaction but was nontoxic to mice. The identity of LPS was verified by alkali and lysozyme treatment. The results suggest that S. calcicola is one of the principal sources of endotoxins in water systems using open finished-water reservoirs.
摘要
宾夕法尼亚州西南部饮用水系统中最常见的蓝藻是钙裂须藻。通过热酚 - 水萃取法从该物种中分离出脂多糖（LPS）。多糖部分由氨基葡萄糖、半乳糖、葡萄糖、甘露糖、木糖和鼠李糖组成。脂质A部分含有β - 羟基月桂酸、肉豆蔻酸、十五烷酸、棕榈酸、β - 羟基棕榈酸、硬脂酸、油酸和亚油酸。与从肠杆菌科分离出的许多LPS不同，主要成分不是β - 羟基肉豆蔻酸而是β - 羟基棕榈酸。该LPS可诱导鲎试剂凝胶化和施瓦茨曼反应，但对小鼠无毒。通过碱处理和溶菌酶处理验证了LPS的特性。结果表明，钙裂须藻是使用开放式成品水库的水系统中内毒素的主要来源之一。
相似文献
1
Composition and biological properties of lipopolysaccharides isolated from Schizothrix calcicola (Ag.) Gomont (Cyanobacteria).从嗜钙裂须藻（Ag.）Gomont（蓝细菌）中分离得到的脂多糖的组成和生物学特性。
Appl Environ Microbiol. 1979 Sep;38(3):471-7. doi: 10.1128/aem.38.3.471-477.1979.
2
Production and properties of cyanobacterial endotoxins.蓝藻内毒素的产生与特性
Appl Environ Microbiol. 1982 Jan;43(1):104-9. doi: 10.1128/aem.43.1.104-109.1982.
3
Isolation and characterization of lipopolysaccharides from cell walls of blue-green algae of the genus Phormidium.席藻属蓝绿藻细胞壁脂多糖的分离与特性研究
J Bacteriol. 1977 Apr;130(1):1-3. doi: 10.1128/jb.130.1.1-3.1977.
4
Lipopolysaccharide containing L-acofriose in the filamentous blue-green alga Anabaena variabilis.在丝状蓝藻多变鱼腥藻中含有L-阿可弗糖的脂多糖。
J Bacteriol. 1974 Nov;120(2):672-8. doi: 10.1128/jb.120.2.672-678.1974.
5
Chemical composition and biological properties of a lipopolysaccharide from Bacteroides intermedius.中间普氏菌脂多糖的化学成分与生物学特性
Acta Pathol Microbiol Immunol Scand B. 1986 Aug;94(4):265-71. doi: 10.1111/j.1699-0463.1986.tb03051.x.
6
Lipopolysaccharides of the cyanobacterium Microcystis aeruginosa.
Eur J Biochem. 1983 Dec 1;137(1-2):333-6. doi: 10.1111/j.1432-1033.1983.tb07833.x.
7
Isolation and chemical composition of lipopolysaccharide from Campylobacter jejuni.空肠弯曲菌脂多糖的分离及化学成分分析
Acta Pathol Microbiol Immunol Scand B. 1982 Apr;90(2):135-9. doi: 10.1111/j.1699-0463.1982.tb00095.x.
8
Chemical properties of lipopolysaccharide-like substance (LLS) extracted from Leptospira interrogans serovar canicola strain Moulton.从问号钩端螺旋体犬型菌株莫尔顿中提取的脂多糖样物质（LLS）的化学性质。
Microbiol Immunol. 1987;31(8):717-25. doi: 10.1111/j.1348-0421.1987.tb03133.x.
9
Immunochemical and toxic properties of the lipopolysaccharides from highly and low virulent Pseudomonas aeruginosa strains.高毒力和低毒力铜绿假单胞菌菌株脂多糖的免疫化学和毒性特性。
J Hyg Epidemiol Microbiol Immunol. 1974;18(4):425-8.
10
Chemical characterization and biological properties of lipopolysaccharides isolated from smooth and rough strains of Brucella abortus.从流产布鲁氏菌光滑型菌株和粗糙型菌株中分离出的脂多糖的化学特性及生物学特性
Infect Immun. 1979 Mar;23(3):811-8. doi: 10.1128/iai.23.3.811-818.1979.
引用本文的文献
1
Biomass-Derived Nanoporous Carbon Honeycomb Monoliths for Environmental Lipopolysaccharide Adsorption from Aqueous Media.用于从水介质中吸附环境脂多糖的生物质衍生纳米多孔碳蜂窝整料
Int J Mol Sci. 2025 Jan 23;26(3):952. doi: 10.3390/ijms26030952.
2
The role of the protective shield against UV-C radiation and its molecular interactions in Nostoc species (Cyanobacteria).UV-C 辐射防护盾的作用及其在念珠藻属（蓝藻）中的分子相互作用。
Sci Rep. 2024 Aug 20;14(1):19258. doi: 10.1038/s41598-024-70002-8.
3
Influencing factors and health risk assessment of microcystins in the Yongjiang river (China) by Monte Carlo simulation.基于蒙特卡洛模拟的中国邕江微囊藻毒素影响因素及健康风险评估
PeerJ. 2018 Nov 16;6:e5955. doi: 10.7717/peerj.5955. eCollection 2018.
4
Lipid A structural modifications in extreme conditions and identification of unique modifying enzymes to define the Toll-like receptor 4 structure-activity relationship.在极端条件下脂 A 结构的修饰以及鉴定独特的修饰酶来定义 Toll 样受体 4 的结构-活性关系。
Biochim Biophys Acta Mol Cell Biol Lipids. 2017 Nov;1862(11):1439-1450. doi: 10.1016/j.bbalip.2017.01.004. Epub 2017 Jan 17.
5
Structure and Effects of Cyanobacterial Lipopolysaccharides.蓝藻脂多糖的结构与作用
Mar Drugs. 2015 Jul 7;13(7):4217-30. doi: 10.3390/md13074217.
6
Slr2019, lipid A transporter homolog, is essential for acidic tolerance in Synechocystis sp. PCC6803.Slr2019，脂多糖A转运蛋白同源物，对集胞藻PCC6803的耐酸性至关重要。
Photosynth Res. 2015 Aug;125(1-2):267-77. doi: 10.1007/s11120-015-0129-6. Epub 2015 Mar 31.
7
Biosynthesis and function of extracellular glycans in cyanobacteria.蓝藻细胞外聚糖的生物合成与功能
Life (Basel). 2015 Jan 12;5(1):164-80. doi: 10.3390/life5010164.
8
Structure of compositionally simple lipopolysaccharide from marine synechococcus.来自海洋聚球藻的成分简单的脂多糖结构
J Bacteriol. 2009 Sep;191(17):5499-509. doi: 10.1128/JB.00121-09. Epub 2009 Jul 6.
9
A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression.一种蓝藻脂多糖拮抗剂可预防内毒素休克，并阻断细胞因子表达所需的TLR4持续刺激。
J Exp Med. 2006 Jun 12;203(6):1481-92. doi: 10.1084/jem.20060136. Epub 2006 May 22.
10
Cyanobacterial lipopolysaccharides and human health - a review.蓝藻脂多糖与人类健康——综述
Environ Health. 2006 Mar 24;5:7. doi: 10.1186/1476-069X-5-7.
本文引用的文献
1
An Epidemiological Study of Suspected Water-borne Gastroenteritis.疑似水源性肠胃炎的流行病学研究
Am J Public Health Nations Health. 1931 Nov;21(11):1227-35. doi: 10.2105/ajph.21.11.1227.
2
PUBLIC HEALTH ENGINEERING: EPIDEMIC OF INTESTINAL DISORDERS IN CHARLESTION, W. VA., OCCURRING SIMULTANEOUSLY WITH UNPRECEDENTED WATER SUPPLY CONDITIONS.公共卫生工程：西弗吉尼亚州查尔斯顿市肠道疾病流行，与史无前例的供水状况同时发生。
Am J Public Health Nations Health. 1931 Feb;21(2):198-200. doi: 10.2105/ajph.21.2.198.
3
On the immunochemistry of O antigens of Enterobacteriaceae. I. Analysis of the sugar component of Salmonella O antigens.关于肠杆菌科O抗原的免疫化学。I. 沙门氏菌O抗原糖成分的分析。
Zentralbl Bakteriol. 1960 May;178:442-58.
4
The identification of 4-deoxy-d-arabino-hexose as a constituent in lipopolysaccharides of four Citrobacter species.4-脱氧-D-阿拉伯己糖作为四种柠檬酸杆菌属细菌脂多糖成分的鉴定。
Eur J Biochem. 1970 Oct;16(2):284-8. doi: 10.1111/j.1432-1033.1970.tb01083.x.
5
On the taxonomy, ecology and immunochemistry of genus Citrobacter.关于柠檬酸杆菌属的分类学、生态学和免疫化学
J Hyg Epidemiol Microbiol Immunol. 1971;15(4):366-74.
6
Seasonal gastroenteritis and malabsorption at an American military base in the Philippines. I. Clinical and epidemiologic investigations of the acute illness.菲律宾一个美国军事基地的季节性肠胃炎和吸收不良。I. 急性疾病的临床和流行病学调查。
Am J Epidemiol. 1972 Feb;95(2):111-27. doi: 10.1093/oxfordjournals.aje.a121376.
7
Identification and analysis of a lipopolysaccharide in cell walls of the blue-green alga Anacystis nidulans.蓝绿藻集胞藻细胞壁中脂多糖的鉴定与分析。
Arch Mikrobiol. 1970;71(1):89-98. doi: 10.1007/BF00412238.
8
Immunochemical studies on Citrobacter O antigens (lipopolysaccharides).关于柠檬酸杆菌O抗原（脂多糖）的免疫化学研究。
Eur J Biochem. 1971 May 28;20(2):237-44. doi: 10.1111/j.1432-1033.1971.tb01386.x.
9
Isolation of D-galacturonic acid 1-phosphate from hydrolysates of cell wall lipopolysaccharide extracted from Xanthomonas campestris.从野油菜黄单胞菌提取的细胞壁脂多糖水解物中分离出1-磷酸-D-半乳糖醛酸。
J Bacteriol. 1968 Mar;95(3):782-6. doi: 10.1128/jb.95.3.782-786.1968.
10
[Contribution to the diagnosis of shock: demonstration of endotoxin and mucopeptide by the Limulus polyphemus-lysate test (author's transl)].[对休克诊断的贡献：用鲎试剂试验检测内毒素和粘肽（作者译）]
Klin Wochenschr. 1974 Feb 15;52(4):175-8. doi: 10.1007/BF01614393.
Zotero 插件