从嗜钙裂须藻（Ag.）Gomont（蓝细菌）中分离得到的脂多糖的组成和生物学特性。
Composition and biological properties of lipopolysaccharides isolated from Schizothrix calcicola (Ag.) Gomont (Cyanobacteria).
作者信息
Keleti G, Sykora J L, Lippy E C, Shapiro M A
出版信息
Appl Environ Microbiol. 1979 Sep;38(3):471-7. doi: 10.1128/aem.38.3.471-477.1979.
Abstract
The most common cyanobacterium contaminating drinking water systems in southwestern Pennsylvania is Schizothrix calcicola. Lipoplysaccharides (LPS) were isolated from this species by hot phenol-water extraction. The polysaccharide moiety was composed of glucosamine, galactose, glucose, mannose, xylose and rhamnose. The lipid A part contained beta-hydroxylauric, myristic, pentadecanoic, palmitic, beta-hydroxypalmitic, stearic, oleic, and linoleic acids. In contrast to many LPS isolated from Enterobacteriaceae, the dominant component was not beta-hydroxymyristic but beta-hydroxypalmitic acid. The LPS induced Limulus lysate gelation and Schwartzman reaction but was nontoxic to mice. The identity of LPS was verified by alkali and lysozyme treatment. The results suggest that S. calcicola is one of the principal sources of endotoxins in water systems using open finished-water reservoirs.
摘要
宾夕法尼亚州西南部饮用水系统中最常见的蓝藻是钙裂须藻。通过热酚 - 水萃取法从该物种中分离出脂多糖（LPS）。多糖部分由氨基葡萄糖、半乳糖、葡萄糖、甘露糖、木糖和鼠李糖组成。脂质A部分含有β - 羟基月桂酸、肉豆蔻酸、十五烷酸、棕榈酸、β - 羟基棕榈酸、硬脂酸、油酸和亚油酸。与从肠杆菌科分离出的许多LPS不同，主要成分不是β - 羟基肉豆蔻酸而是β - 羟基棕榈酸。该LPS可诱导鲎试剂凝胶化和施瓦茨曼反应，但对小鼠无毒。通过碱处理和溶菌酶处理验证了LPS的特性。结果表明，钙裂须藻是使用开放式成品水库的水系统中内毒素的主要来源之一。
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