Andersen Søren S L, Wittmann Torsten
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200, USA.
Bioessays. 2002 Apr;24(4):305-7. doi: 10.1002/bies.10084.
The transition from interphase to mitosis is marked by a dramatic change in microtubule dynamics resulting in the reorganization of the microtubule network that culminates in mitotic spindle formation. While the molecular basis for this change in microtubule organization remains obscure, it is currently thought that a balance in the activity of microtubule stabilizing and destabilizing factors regulates how dynamic cellular microtubules are. By mixing the microtubule stabilizer XMAP215 and the microtubule destabilizer XKCM1, reconstitution of in vivo-like microtubule dynamics has now been achieved in vitro.
从间期到有丝分裂的转变以微管动力学的显著变化为标志，这种变化导致微管网络的重组，最终形成有丝分裂纺锤体。虽然微管组织这一变化的分子基础仍不清楚，但目前认为，微管稳定因子和去稳定因子活性的平衡调节着细胞微管的动态变化。通过混合微管稳定剂XMAP215和微管去稳定剂XKCM1，现已在体外实现了类似体内的微管动力学重建。
