XKCM1：一种非洲爪蟾驱动蛋白相关蛋白，在有丝分裂纺锤体组装过程中调节微管动力学。

XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly.

作者信息

Walczak C E, Mitchison T J, Desai A

机构信息

Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143-0450, USA.

出版信息

Cell. 1996 Jan 12;84(1):37-47. doi: 10.1016/s0092-8674(00)80991-5.

DOI:10.1016/s0092-8674(00)80991-5

PMID:8548824

Abstract

We isolated a cDNA clone encoding a kinesin-related protein, which we named XKCM1. Antibodies to XKCM1 stain mitotic centromeres and spindle poles. Immunodepletion and antibody addition experiments in an in vitro spindle assembly assay show that XKCM1 is required for both establishment and maintenance of mitotic spindles. The structures that form in the absence of XKCM1 contain abnormally long microtubules. This long microtubule defect can be rescued by the addition of purified XKCM1 protein. Analysis of microtubule dynamics in a clarified mitotic extract reveals that loss of XKCM1 function causes a 4-fold suppression in the catastrophe frequency. XKCM1 thus exhibits a novel activity for a kinesin-related protein by promoting microtubule depolymerization during mitotic spindle assembly.

摘要

我们分离出了一个编码驱动蛋白相关蛋白的cDNA克隆，将其命名为XKCM1。针对XKCM1的抗体可对有丝分裂着丝粒和纺锤体极进行染色。体外纺锤体组装试验中的免疫去除和抗体添加实验表明，有丝分裂纺锤体的建立和维持都需要XKCM1。在没有XKCM1的情况下形成的结构包含异常长的微管。添加纯化的XKCM1蛋白可以挽救这种长微管缺陷。对澄清的有丝分裂提取物中的微管动力学分析表明，XKCM1功能丧失会导致灾难频率降低4倍。因此，XKCM1通过在有丝分裂纺锤体组装过程中促进微管解聚，展现出了一种驱动蛋白相关蛋白的新活性。