Lindeberg Jonas, Mattsson Ragnar, Ebendal Ted
Department of Neuroscience, Box 587, BMC, Uppsala University, S-751 23 Uppsala, Sweden.
J Neurosci Res. 2002 Apr 15;68(2):248-53. doi: 10.1002/jnr.10213.
We have developed a transgenic mouse expressing the Cre recombinase under control of a tetracycline-responsive promoter. Using a CamKIIalpha-driven tTA transgenic strain and a lacZ reporter mouse, we obtained the expected neuronal pattern of recombination in the olfactory lobe, cortex, striatum, hippocampus and Purkinje cells. Moreover, recombination can be completely abolished by feeding the mice doxycycline in their drinking water. We also show that it is possible to get a different pattern of recombination by changing the timing of the doxycycline-mediated shutdown of Cre expression. By starting the doxycycline treatment at birth, we restrict recombination to striatum only. This approach should be applicable to other inducible transgenic strains, thus increasing the number of available tissue-specific patterns for conditional knockouts. Also, our tetO-Cre transgene can be combined with any of the increasing number of tetracycline transactivator transgenic strains to direct specifically inducible genomic recombination to several areas of the brain.
我们构建了一种在四环素反应性启动子控制下表达Cre重组酶的转基因小鼠。利用一种由CaMKIIα驱动的tTA转基因品系和一只lacZ报告基因小鼠，我们在嗅叶、皮质、纹状体、海马体和浦肯野细胞中获得了预期的神经元重组模式。此外，通过在小鼠饮用水中添加强力霉素，可以完全消除重组。我们还表明，通过改变强力霉素介导的Cre表达关闭时间，可以获得不同的重组模式。在出生时开始强力霉素治疗，我们将重组限制在纹状体。这种方法应该适用于其他可诱导的转基因品系，从而增加用于条件性基因敲除的可用组织特异性模式的数量。此外，我们的tetO-Cre转基因可以与越来越多的四环素反式激活因子转基因品系中的任何一种结合，以将特异性诱导的基因组重组定向到大脑的几个区域。
