Key Laboratory of Regenerative Medicine of Ministry of Education, College of Life Science and Technology, Jinan University, Guangzhou, China.
State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, China; School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China.
J Biol Chem. 2021 Jan-Jun;296:100509. doi: 10.1016/j.jbc.2021.100509. Epub 2021 Mar 4.
Site-specific recombinases (SSRs) are invaluable genome engineering tools that have enormously boosted our understanding of gene functions and cell lineage relationships in developmental biology, stem cell biology, regenerative medicine, and multiple diseases. However, the ever-increasing complexity of biomedical research requires the development of novel site-specific genetic recombination technologies that can manipulate genomic DNA with high efficiency and fine spatiotemporal control. Here, we review the latest innovative strategies of the commonly used Cre-loxP recombination system and its combinatorial strategies with other site-specific recombinase systems. We also highlight recent progress with a focus on the new generation of chemical- and light-inducible genetic systems and discuss the merits and limitations of each new and established system. Finally, we provide the future perspectives of combining various recombination systems or improving well-established site-specific genetic tools to achieve more efficient and precise spatiotemporal genetic manipulation.
位点特异性重组酶（SSR）是一种极具价值的基因组工程工具，极大地促进了我们对发育生物学、干细胞生物学、再生医学和多种疾病中基因功能和细胞谱系关系的理解。然而，生物医学研究的复杂性不断增加，需要开发新型的位点特异性基因重组技术，以实现高效和精细的时空控制对基因组 DNA 的操作。在这里，我们回顾了常用的 Cre-loxP 重组系统及其与其他位点特异性重组系统的组合策略的最新创新策略。我们还重点介绍了最新进展，关注新一代化学诱导和光诱导遗传系统，并讨论了每个新系统和已建立系统的优点和局限性。最后，我们提供了结合各种重组系统或改进成熟的位点特异性遗传工具以实现更高效和精确的时空遗传操作的未来展望。
