Ahmed Shahira Abdelaziz Ali, Quattrocchi Annalisa, Elzagawy Sherine M, Karanis Panagiotis, Gad Samer Eid Mohamed
Department of Parasitology, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt.
Department of Primary Care and Population Health, University of Nicosia Medical School, 21 Ilia Papakyriakou, 2414 Engomi, Nicosia CY-1700, Cyprus.
Diagnostics (Basel). 2023 Aug 1;13(15):2557. doi: 10.3390/diagnostics13152557.
(1) Background: The wet mount staining technique is a simple, economical, and rapid method for detecting parasitic stages. The objective of the current study was to evaluate wet mount diagnostic stains for infection in human faecal samples and to compare the sensitivity and qualitative performance of toluidine blue (TolB) and modified Ziehl-Neelsen (mZN) stain. (2) Methods: The collection, purification, and molecular amplification of oocysts were performed. TolB, malachite green, trypan blue, and crystal violet were evaluated qualitatively to diagnose oocysts. The outperforming stain was compared to mZN using a quantitative evaluation and qualitative scoring system. (3) Results: The oocysts of were effectively purified and utilized for spiking. TolB was the most effective diagnostic stain among wet mount stains for detecting oocysts. TolB outperformed mZN in terms of sensitivity, with 100% versus 79% at a concentration of 10 and 49% versus 23% at a concentration of 10. TolB had the most favourable qualitative stain characteristics against mZN; however, sample freshness and being a temporary stain were crucial considerations. (4) Conclusions: This study emphasizes that TolB, as a routine wet mount technique for screening infection, will provide a more sensitive and faster method than mZN staining.
(1) 背景：湿片染色技术是一种检测寄生虫阶段的简单、经济且快速的方法。本研究的目的是评估用于检测人类粪便样本中感染情况的湿片诊断染色剂，并比较甲苯胺蓝（TolB）和改良齐-尼氏（mZN）染色的敏感性和定性性能。(2) 方法：进行了卵囊的收集、纯化和分子扩增。对甲苯胺蓝、孔雀绿、台盼蓝和结晶紫进行定性评估以诊断卵囊。使用定量评估和定性评分系统将表现最佳的染色剂与改良齐-尼氏染色进行比较。(3) 结果：的卵囊得到有效纯化并用于加样。在用于检测卵囊的湿片染色剂中，甲苯胺蓝是最有效的诊断染色剂。在敏感性方面，甲苯胺蓝优于改良齐-尼氏染色，在浓度为10时分别为100%对79%，在浓度为10时分别为49%对23%。与改良齐-尼氏染色相比，甲苯胺蓝具有最有利的定性染色特征；然而，样本新鲜度和作为临时染色剂是关键考虑因素。(4) 结论：本研究强调，甲苯胺蓝作为筛查感染的常规湿片技术，将提供一种比改良齐-尼氏染色更敏感、更快的方法。
