Chu Karen K, Davidson Donald J, Halsey T Keith, Chung Jacqueline W, Speert David P
Department of Paediatrics, University of British Columbia, Vancouver, British Columbia, Canada.
Infect Immun. 2002 May;70(5):2715-20. doi: 10.1128/IAI.70.5.2715-2720.2002.
Cystic fibrosis patients infected with strains from different genomovars of the Burkholderia cepacia complex can experience diverse clinical outcomes. To identify genomovar-specific determinants that might be responsible for these differences, we developed a pulmonary model of infection in BALB/c mice. Mice were rendered leukopenic by administration of cyclophosphamide prior to intranasal challenge with 1.6 x 10(4) bacteria. Five of six genomovar II strains persisted at stable numbers in the lungs until day 16 with minimal toxicity, whereas zero of seven genomovar III strains persisted but resulted in variable toxicity. We have developed a chronic pulmonary model of B. cepacia infection which reveals differences among genomovars in terms of clinical infection outcome.
感染了洋葱伯克霍尔德菌复合体不同基因变种菌株的囊性纤维化患者可能会经历不同的临床结果。为了确定可能导致这些差异的基因变种特异性决定因素，我们在BALB/c小鼠中建立了一种肺部感染模型。在用1.6×10⁴个细菌进行鼻内攻击之前，通过给予环磷酰胺使小鼠白细胞减少。六个基因变种II菌株中的五个在肺部以稳定数量持续存在至第16天，毒性最小，而七个基因变种III菌株中没有一个持续存在，但导致了不同程度的毒性。我们已经建立了一种洋葱伯克霍尔德菌感染的慢性肺部模型，该模型揭示了不同基因变种在临床感染结果方面的差异。
