Olive Colleen, Batzloff Michael R, Horváth Anikó, Wong Allan, Clair Timothy, Yarwood Penny, Toth Istvan, Good Michael F
Division of Infectious Diseases and Immunology, Cooperative Research Centre for Vaccine Technology, The Queensland Institute of Medical Research, Brisbane, Queensland 4029, Australia.
Infect Immun. 2002 May;70(5):2734-8. doi: 10.1128/IAI.70.5.2734-2738.2002.
The study reported here investigated the immunogenicity and protective potential of a lipid core peptide (LCP) construct containing a conserved region determinant of M protein, defined as peptide J8. Parenteral immunization of mice with LCP-J8 led to the induction of high-titer serum immunoglobulin G J8-specific antibodies when the construct was coadministered with complete Freund's adjuvant (CFA) or administered alone. LCP-J8 in CFA had significantly enhanced immunogenicity compared with the monomeric peptide J8 given in CFA. Moreover, LCP-J8/CFA and LCP-J8 antisera opsonized four different group A streptococcal (GAS) strains, and the antisera did not cross-react with human heart tissue proteins. These data indicate the potential of an LCP-based M protein conserved region GAS vaccine in the induction of broadly protective immune responses in the absence of a conventional adjuvant.
本文报道的研究调查了一种包含M蛋白保守区域决定簇（定义为肽J8）的脂质核心肽（LCP）构建体的免疫原性和保护潜力。当该构建体与完全弗氏佐剂（CFA）共同给药或单独给药时，用LCP-J8对小鼠进行肠胃外免疫可诱导产生高滴度的血清免疫球蛋白G J8特异性抗体。与CFA中给予的单体肽J8相比，CFA中的LCP-J8具有显著增强的免疫原性。此外，LCP-J8/CFA和LCP-J8抗血清调理了四种不同的A组链球菌（GAS）菌株，且该抗血清与人心脏组织蛋白无交叉反应。这些数据表明基于LCP的M蛋白保守区域GAS疫苗在无传统佐剂的情况下诱导广泛保护性免疫反应的潜力。
