Tan Guolin, Xiao Jianyun, Tian Yongqian, Dong Longnwen, Jiang Nin, Zhan Fenfang, Li Guiyuan
Department of Otolaryngology, Third Affiliated Hospital, Hunan Medical University, Hunan, Changsha, China.
Otolaryngol Head Neck Surg. 2002 Mar;126(3):296-300. doi: 10.1067/mhn.2002.123046.
Our goal was to better define the extent and specificity of deletion in the 7q32-qter chromosomal region in nasopharyngeal carcinoma (NPC).
Polymerase chain reaction-based deletion analysis was performed on DNA samples from 24 paired NPCs and corresponding germlines using 13 microsatellite markers mapped to chromosome subbands 7q31.3-q36.
Loss of heterozygosity of at least 1 marker in this interval was found in 18 (75%) of 24 tumor specimens. Particularly frequent allelic losses were identified at 5 loci: D7S495 (46%), D7S509 (42%), D7S500 (45%), D7S631 (30%), and D7S514 (35%). Two shortest regions of overlap could be identified in this interval, although the most common shortest region of overlap appeared to lie around D7S500 between but not including D7S631 and D7S495, on chromosome subband 7q32.
These results suggest that at least 2 putative tumor suppressor genes important in the pathogenesis of NPC are present in the examined interval, an interval that has also been found to harbor deletions in breast and prostate carcinomas.
我们的目标是更精确地界定鼻咽癌（NPC）中7q32 - qter染色体区域缺失的范围和特异性。
使用定位到染色体亚带7q31.3 - q36的13个微卫星标记，对来自24对NPC及其相应种系的DNA样本进行基于聚合酶链反应的缺失分析。
在24个肿瘤标本中的18个（75%）中发现该区间至少有1个标记的杂合性缺失。在5个位点发现等位基因缺失尤为频繁：D7S495（46%）、D7S509（42%）、D7S500（45%）、D7S631（30%）和D7S514（35%）。在此区间可确定两个最短重叠区域，尽管最常见的最短重叠区域似乎位于7q32染色体亚带上D7S500周围，介于但不包括D7S631和D7S495之间。
这些结果表明，在所检测的区间内至少存在2个在NPC发病机制中起重要作用的假定肿瘤抑制基因，该区间在乳腺癌和前列腺癌中也发现有缺失。
