Ge Zhi-Jun, Tan Yong-Fei, Zhao Yan-Ping, Cui Guo-Xing
Department of Anaesthesiology, Affiliated Hospital of Jiangsu University, Yixing People's Hospital, Yixing City, Jiangsu, China.
Clin Exp Pharmacol Physiol. 2008 Oct;35(10):1245-51. doi: 10.1111/j.1440-1681.2008.05001.x. Epub 2008 Jul 9.
The aim of the present study was to investigate the contribution of spinal nitric oxide (NO) to the antinociceptive effects of emulsified isofluane in rats. The formalin test was used to assess nociceptive responses. Immunocytochemistry and histochemistry were performed to determine the effects of emulsified isoflurane on formalin-induced changes in Fos-like immunoreactive (Fos-LI)- and nicotinamide adenine dinucleotide phosphatediaphorase (NADPH-d)-positive neurons, respectively. The results showed that emulsified isofluane, administered intraperitoneally, significantly decreased the formalin-induced paw licking time and that this was attenuated by pretreatment with intrathecal injection of the NO precursor L-arginine. Furthermore, Fos-LI- and NADPH-d-positive neurons were mainly found in the ipsilateral dorsal horn after injection of formalin, some of which were Fos-LI/NADPH-d double-labelled neurons. Administration of emulsified isofluane significantly decreased Fos-LI- and NADPH-d-positive, as well as Fos-LI/NADPH-d double-labelled, neurons. Finally, emulsified isofluane produced a significant reduction of NOS activity and a decrease of NO production in the spinal cord of formalin-treated rats. In conclusion, the results suggest that inhibition of spinal NO production contributes to the antinociceptive effects of emulsified isofluane on formalin-induced pain in rats.
本研究的目的是探讨脊髓一氧化氮（NO）对乳化异氟烷在大鼠体内抗伤害感受作用的贡献。采用福尔马林试验评估伤害感受反应。分别进行免疫细胞化学和组织化学检测，以确定乳化异氟烷对福尔马林诱导的Fos样免疫反应性（Fos-LI）和烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）阳性神经元变化的影响。结果表明，腹腔注射乳化异氟烷可显著缩短福尔马林诱导的舔足时间，而鞘内注射NO前体L-精氨酸预处理可减弱这种作用。此外，注射福尔马林后，Fos-LI和NADPH-d阳性神经元主要位于同侧背角，其中一些是Fos-LI/NADPH-d双标神经元。给予乳化异氟烷可显著减少Fos-LI和NADPH-d阳性以及Fos-LI/NADPH-d双标神经元的数量。最后，乳化异氟烷可显著降低福尔马林处理大鼠脊髓中的一氧化氮合酶（NOS）活性并减少NO生成。总之，结果表明抑制脊髓NO生成有助于乳化异氟烷对大鼠福尔马林诱导疼痛的抗伤害感受作用。
