Sonna Larry A, Gaffin Stephen L, Pratt Richard E, Cullivan Michael L, Angel Karen C, Lilly Craig M
Thermal and Mountain Medicine Division, US Army Research Institute of Environmental Medicine, Natick, Massachusetts 01760, USA.
J Appl Physiol (1985). 2002 May;92(5):2208-20. doi: 10.1152/japplphysiol.01002.2001.
We studied the effect of heat shock on gene expression by normal human cells. Peripheral blood mononuclear cells (PBMCs) were obtained from healthy adults. Paired samples from each subject were subjected to either 20 min of heat shock (43 degrees C) or control (37 degrees C) conditions and then returned to 37 degrees C. RNA was isolated 160 min later, and five representative samples were analyzed on Affymetrix gene chip arrays containing approximately 12,600 probes. A biologically meaningful effect was defined as a statistically significant, twofold or greater difference in expression of sequences that were detected in all five experiments under control (downregulated sequences) or heat shock (upregulated sequences) conditions. Changes occurred in 395 sequences (227 increased by heat shock, 168 decreased), representing 353 Unigene numbers, in every functional category previously implicated in the heat shock response. By RT-PCR, we confirmed the findings for one upregulated sequence (Rad, a G protein) and one downregulated sequence (osteopontin, a cytokine). We conclude that heat shock causes extensive gene expression changes in PBMCs, affecting all functional categories of the heat shock response.
我们研究了热休克对正常人细胞基因表达的影响。外周血单个核细胞（PBMCs）取自健康成年人。将每个受试者的配对样本分别置于热休克（43摄氏度，20分钟）或对照（37摄氏度）条件下，然后恢复至37摄氏度。160分钟后分离RNA，并在包含约12,600个探针的Affymetrix基因芯片阵列上分析五个代表性样本。具有生物学意义的效应定义为在对照（下调序列）或热休克（上调序列）条件下的所有五个实验中检测到的序列表达存在统计学显著的两倍或更大差异。在先前涉及热休克反应的每个功能类别中，有395个序列发生了变化（热休克使227个增加，168个减少），代表353个单基因编号。通过逆转录聚合酶链反应（RT-PCR），我们证实了一个上调序列（Rad，一种G蛋白）和一个下调序列（骨桥蛋白，一种细胞因子）的研究结果。我们得出结论，热休克会导致PBMCs中广泛的基因表达变化，影响热休克反应的所有功能类别。
