Wambura Casmir, Aoyama Nobuo, Shirasaka Daisuke, Sakai Toshiyuki, Ikemura Takahiro, Sakashita Masanori, Maekawa Shuji, Kuroda Kohei, Inoue Takashi, Ebara Shigeyuki, Miyamoto Masaki, Kasuga Masato
Second department of Internal medicine and Department of Endoscopy, Kobe University School of Medicine, Japan.
Helicobacter. 2002 Apr;7(2):129-38. doi: 10.1046/j.1083-4389.2002.00069.x.
Cyclooxygenase (COX)-2 induced by Helicobacter pylori is thought to enhance gastric carcinogenesis by affecting the maintenance of epithelial homeostasis.
Gastric biopsies from 160 subjects, 97 with nonulcer dyspepsia (47 H. pylori negative, 50 H. pylori positive) and 63 with gastric cancer were examined immunohistochemically for COX-2 expression, cell proliferation and apoptotic indices.
COX-2 expression in corpus was significantly higher in H. pylori positive than in negative non-ulcer dyspepsia (NUD) (p <.05). Regardless of site, gastric cancer subjects had higher COX-2 expression in both antrum and corpus compared with H. pylori negative and positive NUD (p <.005). Proliferation was higher in cancer and H. pylori positive than in negative NUD (p <.0001). Moreover, cancer had enhanced proliferation than H. pylori positive NUD in corpus greater (p =.0454) and antrum lesser (p =.0215) curvatures. Apoptosis was higher in H. pylori positive than in negative NUD (p <.05). However, both had a higher index than the cancer subjects (p <.0001). Apoptosis : proliferation ratio was higher in corpus of H. pylori negative than in positive NUD in greater (p =.0122) and lesser (p =.0009) curvatures. However, both had a higher A:P ratio than cancer cases (p =.0001). A negative correlation between COX-2 expression and A:P ratio was found in corpus greater (r = -.176, p =.0437) and lesser (r = -.188, p =.0312) curvatures.
The expression of COX-2 is associated with disruption in gastric epithelial kinetics and hence may play a role in gastric carcinogenesis.
幽门螺杆菌诱导的环氧化酶（COX）-2被认为通过影响上皮细胞稳态的维持来增强胃癌发生。
对160例受试者的胃活检组织进行免疫组化检查，其中97例为非溃疡性消化不良（47例幽门螺杆菌阴性，50例幽门螺杆菌阳性），63例为胃癌患者，检测COX-2表达、细胞增殖和凋亡指数。
幽门螺杆菌阳性的非溃疡性消化不良（NUD）患者胃体部COX-2表达显著高于阴性患者（p<.05）。无论部位如何，胃癌患者胃窦和胃体部的COX-2表达均高于幽门螺杆菌阴性和阳性的NUD患者（p<.005）。癌症患者和幽门螺杆菌阳性患者的增殖率高于阴性NUD患者（p<.0001）。此外，在胃体大弯侧（p =.0454）和胃窦小弯侧（p =.0215），癌症患者的增殖率高于幽门螺杆菌阳性的NUD患者。幽门螺杆菌阳性患者的凋亡率高于阴性NUD患者（p<.05）。然而，两者的凋亡指数均高于癌症患者（p<.0001）。在胃体大弯侧（p =.0122）和小弯侧（p =.0009），幽门螺杆菌阴性患者的凋亡:增殖比值高于阳性NUD患者。然而，两者的A:P比值均高于癌症患者（p =.0001）。在胃体大弯侧（r = -.176，p =.0437）和小弯侧（r = -.188，p =.0312），COX-2表达与A:P比值呈负相关。
COX-2的表达与胃上皮动力学紊乱有关，因此可能在胃癌发生中起作用。
