Kohlmeyer Ute, Kuballa Jürgen, Jantzen Eckard
GALAB, Max-Planck-Str. 1, D-21502 Geesthacht, Germany.
Rapid Commun Mass Spectrom. 2002;16(10):965-74. doi: 10.1002/rcm.671.
A method using high-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC/ICP-MS) has been developed to determine inorganic arsenic (arsenite, arsenate) along with organic arsenic compounds (monomethylarsonic acid, dimethylarsinic acid, arsenobetaine, arsenocholine, trimethylarsine oxide, tetramethylarsonium ion and several arsenosugars) in fish, mussel, oyster and marine algae samples. The species were extracted by means of a methanol/water mixture and a dispersion unit in 2 min, with extraction efficiencies ranging from 83 to 107% in the different organisms. Up to 17 different species were determined within 15 min on an anion-exchange column, using a nitric acid gradient and an ion-pairing reagent. As all species are shown in one chromatogram, a clear overview of arsenic distribution patterns in different marine organisms is given. Arsenobetaine is the major compound in marine animals whereas arsenosugars and arsenate are dominant in marine algae. The method was validated with CRM DORM-2 (dogfish muscle). Concentrations were within the certified limits and low detection limits of 8 ng g(-1) (arsenite) to 50 ng g(-1) (arsenate) were obtained.
已开发出一种使用高效液相色谱/电感耦合等离子体质谱法（HPLC/ICP-MS）的方法，用于测定鱼类、贻贝、牡蛎和海藻样品中的无机砷（亚砷酸盐、砷酸盐）以及有机砷化合物（一甲基胂酸、二甲基胂酸、砷甜菜碱、砷胆碱、三甲基氧化胂、四甲基砷离子和几种砷糖）。通过甲醇/水混合物和分散装置在2分钟内提取这些物种，不同生物体中的提取效率在83%至107%之间。使用硝酸梯度和离子对试剂，在阴离子交换柱上15分钟内可测定多达17种不同的物种。由于所有物种都显示在一张色谱图中，因此可以清楚地了解不同海洋生物中砷的分布模式。砷甜菜碱是海洋动物中的主要化合物，而砷糖和砷酸盐在海藻中占主导地位。该方法用CRM DORM-2（角鲨肌肉）进行了验证。浓度在认证范围内，获得了8 ng g⁻¹（亚砷酸盐）至50 ng g⁻¹（砷酸盐）的低检测限。
