Lebedev V S, Veselovskiĭ A V, Deĭnega E Iu, Fedorov Iu I
Institute of Energy Problems of Chemical Physics, Russian Academy of Sciences, Leninskii pr. 38/2, Moscow, 117829 Russia.
Biofizika. 2002 Mar-Apr;47(2):295-9.
The role of active oxygen species in the induction of nonselective cationic permeability of the plasma membrane of bacteria Escherichia coli B by the action of Cu2+ ions was studied. It was found that the increase in the amount of active oxygen species in the suspension after treating cells with copper occurred synchronously with the leakage of K+ cations from them. Evidence is presented that active oxygen species formed during the interaction of copper ions with bacteria under aerobic conditions are not involved in the induction of channel conductivity in the membrane. Moreover, the ability of oxygen to protect the membrane from the toxic action of copper was shown, and the activation of membrane damage by external reductants was confirmed. These data suggest that the barrier properties of the membrane are disturbed during the interaction of Cu+ ions with critical targets on the surface, the concentration of Cu+ being determined by all redox processes in the near-membrane space.
研究了活性氧物种在铜离子作用下诱导大肠杆菌B质膜非选择性阳离子通透性中的作用。发现用铜处理细胞后，悬浮液中活性氧物种数量的增加与钾离子从细胞中的泄漏同步发生。有证据表明，在有氧条件下铜离子与细菌相互作用过程中形成的活性氧物种不参与膜通道电导率的诱导。此外，还表明了氧气保护膜免受铜毒性作用的能力，并证实了外部还原剂对膜损伤的激活作用。这些数据表明，在铜离子与表面关键靶点相互作用期间，膜的屏障特性受到干扰，铜离子的浓度由近膜空间中的所有氧化还原过程决定。
