Lecuit Thomas, Samanta Reba, Wieschaus Eric
Howard Hughes Medical Institute, Molecular Biology Department, Washington Road, Princeton University, Princeton, NJ 08544, USA.
Dev Cell. 2002 Apr;2(4):425-36. doi: 10.1016/s1534-5807(02)00141-7.
Cellularization of the Drosophila embryo is a specialized form of cytokinesis that couples membrane growth with the formation of a polarized epithelium. We have identified a gene essential for polarized growth of the plasma membrane during cellularization. In slam mutant embryos, the furrow canal is disorganized, and polarized insertion of transmembrane proteins is disrupted. slam shows a striking developmental induction during the slow phase of cellularization, and Slam protein localizes to the furrow canal and the basal junction. Slam colocalizes with the junctional proteins Arm/beta-catenin, the PDZ domain-containing protein Dlt, and Myosin and is also required for their proper membrane localization. Our results suggest that developmental induction of Slam organizes the polarized growth of membrane via the recruitment of membrane-targeting proteins at adherens junctions.
果蝇胚胎的细胞化是一种特殊形式的胞质分裂，它将细胞膜生长与极化上皮的形成联系起来。我们鉴定出了一个在细胞化过程中对质膜极化生长至关重要的基因。在slam突变体胚胎中，沟道紊乱，跨膜蛋白的极化插入被破坏。slam在细胞化的缓慢阶段表现出显著的发育诱导，并且Slam蛋白定位于沟道和基底连接。Slam与连接蛋白Arm/β-连环蛋白、含PDZ结构域的蛋白Dlt和肌球蛋白共定位，并且它们正确的膜定位也需要Slam。我们的结果表明，Slam的发育诱导通过在黏着连接处募集膜靶向蛋白来组织膜的极化生长。
