Yan Shuling, Großhans Jörg
a Institute for Developmental Biochemistry, Medical School , University of Göttingen , Göttingen , Germany.
Fly (Austin). 2018;12(3-4):191-198. doi: 10.1080/19336934.2018.1520574. Epub 2018 Sep 18.
In this extra view, we comment on our recent work concerning the mRNA localization of the gene slow as molasses (slam). slam is a gene essential for the polarized invagination of the plasma membrane and separation of basal and lateral cortical domains during cellularization as well as for germ cell migration in later embryogenesis. We have demonstrated an intimate relationship between slam RNA and its encoded protein. Slam RNA co-localizes and forms a complex with its encoded protein. Slam mRNA localization not only is required for reaching full levels of functional Slam protein but also depends on Slam protein. The translation of slam mRNA is subject to tight spatio-temporal regulation leading to a rapid accumulation of Slam protein and zygotic slam RNA at the furrow canal. In this extra view, we first discuss the mechanism controlling localization and translation of slam RNA. In addition, we document in detail the maternal and zygotic expression of slam RNA and protein and provide data for a function in membrane stabilization. Furthermore, we mapped the region of Slam protein mediating cortical localization in cultured cells.
在这篇补充观点文章中，我们对近期关于“慢如糖浆”（slam）基因的mRNA定位的研究工作进行评论。slam基因对于细胞膜的极化内陷以及细胞化过程中基底和侧面皮质结构域的分离，以及后期胚胎发育中的生殖细胞迁移至关重要。我们已经证明了slam RNA与其编码蛋白之间存在密切关系。slam RNA与其编码蛋白共定位并形成复合物。slam mRNA定位不仅是达到功能完整水平的Slam蛋白所必需的，而且还依赖于Slam蛋白。slam mRNA的翻译受到严格的时空调控，导致Slam蛋白和合子型slam RNA在沟道处快速积累。在这篇补充观点文章中，我们首先讨论控制slam RNA定位和翻译的机制。此外，我们详细记录了slam RNA和蛋白的母体和合子型表达，并提供了其在膜稳定中的功能数据。此外，我们绘制了在培养细胞中介导皮质定位的Slam蛋白区域。
