通过细胞因子流式细胞术检测S抗原特异性效应T细胞活化。
S antigen specific effector T cell activation detected by cytokine flow cytometry.
作者信息
Morgan J P, Robins R A, Dua H S, Tighe P J
机构信息
The Larry Donoso Research Laboratory, Division of Ophthalmology and Visual Sciences, Eye Ear Nose and Throat Centre, Queen's Medical Centre, University of Nottingham, Nottingham NG7 2UH, UK.
出版信息
Br J Ophthalmol. 2002 May;86(5):517-20. doi: 10.1136/bjo.86.5.517.
BACKGROUND/AIMS: Effector T cell activation is particularly important in the initiation of autoimmune uveitis. This pilot study seeks to demonstrate activation of human peripheral effector T cells in response to the uveitis candidate autoantigen, retinal S antigen (SAg), using cytokine flow cytometry (CFC).
METHODS
Peripheral blood mononuclear cell (PBMC) suspensions from uveitis patients and controls were stimulated with bovine SAg. Activation responses were detected by CFC.
RESULTS
Electronic gating enabled analysis of CD69+, IFN-gamma+ CD4+ lymphocytes. An SAg specific response was detectable in four of 13 patients and four of eight controls.
CONCLUSION
SAg specific, peripheral, effector T cell activation can be detected by CFC. Similar levels of responsiveness were seen in patient and control groups. More detailed cytokine profiling may demonstrate functional differences between the groups.
背景/目的：效应T细胞激活在自身免疫性葡萄膜炎的发病中尤为重要。本初步研究旨在利用细胞因子流式细胞术（CFC）证明人类外周效应T细胞对葡萄膜炎候选自身抗原视网膜S抗原（SAg）产生的激活反应。
方法
用牛SAg刺激葡萄膜炎患者和对照者的外周血单个核细胞（PBMC）悬液。通过CFC检测激活反应。
结果
电子门控能够分析CD69 +、IFN-γ + CD4 +淋巴细胞。在13例患者中的4例以及8例对照者中的4例中可检测到SAg特异性反应。
结论
通过CFC可检测到SAg特异性外周效应T细胞激活。患者组和对照组的反应性水平相似。更详细的细胞因子谱分析可能会显示两组之间的功能差异。
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