Calder V L, Shaer B, Muhaya M, Mclauchlan M, Pearson R V, Jolly G, Towler H M, Lightman S
Department of Clinical Ophthalmology, Institute of Ophthalmology, UCL, London, UK.
Invest Ophthalmol Vis Sci. 1999 Aug;40(9):2019-24.
To compare cell types and cytokines in aqueous humor from patients with uveitis either occurring in association with a systemic disease or apparently isolated and not associated with a systemic disease.
Cells were collected by centrifugation of fresh aqueous humor from uveitis and controls, and immunofluorescence techniques were performed with markers for T cells, B cells, and monocytes. Cytokines were measured in the aqueous supernatants, and serum samples were assayed for soluble interleukin-2 receptors.
When aqueous samples from idiopathic uveitis were compared with those from uveitis associated with a systemic disease, there were increases in CD3+, CD4+ (p = 0.001), and activated CD4+ T cells (p = 0.02) and a decrease in B cells (p = 0.0013). This was not reflected in the peripheral blood where there were no differences in the cell types or in soluble interleukin-2 receptor levels. No cells were obtainable from control aqueous. Interleukins-10 and -12, interferon-gamma, and transforming growth factor-beta2 were detected in aqueous supernatants. Interleukin-10 was reduced (p = 0.024) in uveitis in comparison with controls.
The results suggest a selective recruitment of CD4+ T cells within aqueous humor but only in idiopathic uveitis. In both disease groups there was a decrease in the immunoregulatory cytokine interleukin-10, which might enable an immune response to occur in an otherwise highly immunosuppressive microenvironment. Increases in activated CD4+ T cells combined with depressed interleukin-10 levels could partially explain why, for example, in acute anterior uveitis, the inflammatory disease is often more severe.
比较与全身性疾病相关或明显孤立且与全身性疾病无关的葡萄膜炎患者房水中的细胞类型和细胞因子。
通过离心收集葡萄膜炎患者和对照组的新鲜房水细胞，并用T细胞、B细胞和单核细胞标志物进行免疫荧光技术检测。检测房水上清液中的细胞因子，并检测血清样本中的可溶性白细胞介素-2受体。
将特发性葡萄膜炎的房水样本与与全身性疾病相关的葡萄膜炎的房水样本进行比较时，CD3 +、CD4 +（p = 0.001）和活化的CD4 + T细胞（p = 0.02）增加，B细胞减少（p = 0.0013）。这在外周血中未得到体现，外周血中的细胞类型或可溶性白细胞介素-2受体水平没有差异。对照组房水未获得细胞。在房水上清液中检测到白细胞介素-10和-12、干扰素-γ和转化生长因子-β2。与对照组相比，葡萄膜炎患者房水中白细胞介素-10减少（p = 0.024）。
结果表明，仅在特发性葡萄膜炎中，房水内有CD4 + T细胞的选择性募集。在两个疾病组中，免疫调节细胞因子白细胞介素-10均减少，这可能使免疫反应在原本高度免疫抑制的微环境中发生。活化的CD4 + T细胞增加与白细胞介素-10水平降低相结合，可能部分解释了例如急性前葡萄膜炎中炎症性疾病往往更严重的原因。
