来自食酸戴尔福特菌质粒pUO1的携带两个卤乙酸脱卤酶基因的II类缺陷转座子的特性分析。
Characterization of a class II defective transposon carrying two haloacetate dehalogenase genes from Delftia acidovorans plasmid pUO1.
作者信息
Sota Masahiro, Endo Masahiro, Nitta Keiji, Kawasaki Haruhiko, Tsuda Masataka
机构信息
Department of Environmental Simulation, Institute for Environmental Sciences, Obuchi, Rokkasho, Aomori 039-3212, Japan.
出版信息
Appl Environ Microbiol. 2002 May;68(5):2307-15. doi: 10.1128/AEM.68.5.2307-2315.2002.
Abstract
The two haloacetate dehalogenase genes, dehH1 and dehH2, on the 65-kb plasmid pUO1 from Delftia acidovorans strain B were found to be located on transposable elements. The dehH2 gene was carried on an 8.9-kb class I composite transposon (TnHad1) that was flanked by two directly repeated copies of IS1071, IS1071L and IS1071R. The dehH1 gene was also flanked by IS1071L and a truncated version of IS1071 (IS1071N). TnHad1, dehH1, and IS1071N were located on a 15.6-kb class II transposon (TnHad2) whose terminal inverted repeats and res site showed high homology with those of the Tn21-related transposons. TnHad2 was defective in transposition because of its lacking the transposase and resolvase genes. TnHad2 could transpose when the Tn21-encoded transposase and resolvase were supplied in trans. These results demonstrated that Tn Had2 is a defective Tn21-related transposon carrying another class I catabolic transposon.
摘要
从嗜酸戴尔福特菌B菌株的65 kb质粒pUO1上发现的两个卤乙酸脱卤酶基因dehH1和dehH2位于转座元件上。dehH2基因位于一个8.9 kb的I类复合转座子（TnHad1）上，其两侧是IS1071、IS1071L和IS1071R的两个直接重复拷贝。dehH1基因也位于IS1071L和IS1071的一个截短版本（IS1071N）的两侧。TnHad1、dehH1和IS1071N位于一个15.6 kb的II类转座子（TnHad2）上，其末端反向重复序列和res位点与Tn21相关转座子的末端反向重复序列和res位点具有高度同源性。由于缺乏转座酶和解离酶基因，TnHad2在转座方面存在缺陷。当通过反式提供Tn21编码的转座酶和解离酶时，TnHad2可以进行转座。这些结果表明，TnHad2是一个携带另一个I类分解代谢转座子的缺陷型Tn21相关转座子。
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