Franz Charles M A P, Grube Alexandra, Herrmann Anette, Abriouel Hikmate, Stärke Joachim, Lombardi Angiolella, Tauscher Bernhard, Holzapfel Wilhelm H
Institute for Biotechnology and Molecular Biology, Federal Research Centre for Nutrition, D-76131 Karlsruhe, Germany.
Appl Environ Microbiol. 2002 May;68(5):2550-4. doi: 10.1128/AEM.68.5.2550-2554.2002.
The structural genes for the two-peptide bacteriocin enterocin 1071 (Ent1071) in Enterococcus faecalis FAIR-E 309 were cloned. DNA sequence analysis showed that the enterocin 1071A (Ent1071A) peptide of strain FAIR-E 309 differed by two amino acids from the Ent1071A reported for E. faecalis BFE 1071 (E. Balla, L. M. T. Dicks, M. Du Toit, M. J. van der Merwe, and W. H. Holzapfel, Appl. Environ. Microbiol. 66:1298-1304, 2000), while the Ent1071B gene encoded identical peptides in these strains. However, resequencing of ent1071A from E. faecalis BFE 1071 showed that the Ent1071A peptide sequence reported previously was incorrect in two amino acids. Also, ent1071B in E. faecalis FAIR-E 309 encoded a prepeptide that was three amino acids shorter than that previously reported for E. faecalis BFE 1071 Ent1071B. A presumptive immunity gene (eni1071) was located downstream of the bacteriocin structural genes. This gene was cloned into the heterologous host E. faecalis ATCC 19433 and was shown to confer immunity. A truncated ABC transporter gene was located upstream of the Ent1071 structural genes.
克隆了粪肠球菌FAIR-E 309中双肽细菌素肠球菌素1071（Ent1071）的结构基因。DNA序列分析表明，FAIR-E 309菌株的肠球菌素1071A（Ent1071A）肽与报道的粪肠球菌BFE 1071（E. Balla、L. M. T. Dicks、M. Du Toit、M. J. van der Merwe和W. H. Holzapfel，《应用与环境微生物学》66:1298 - 1304，2000）的Ent1071A在两个氨基酸上存在差异，而Ent1071B基因在这些菌株中编码相同的肽。然而，对粪肠球菌BFE 1071的ent1071A重新测序表明，先前报道的Ent1071A肽序列在两个氨基酸上是错误的。此外，粪肠球菌FAIR-E 309中的ent1071B编码一种前肽，其比先前报道的粪肠球菌BFE 1071 Ent1071B的前肽短三个氨基酸。一个推定的免疫基因（eni1071）位于细菌素结构基因的下游。该基因被克隆到异源宿主粪肠球菌ATCC 19433中，并显示出具有免疫功能。一个截短的ABC转运蛋白基因位于Ent1071结构基因的上游。
