基于nifH和cpcBA-IGS核苷酸序列分析对来自不同地理来源的柱孢鱼腥藻（蓝细菌）分离株进行遗传特征分析。
Genetic characterization of Cylindrospermopsis raciborskii (cyanobacteria) isolates from diverse geographic origins based on nifH and cpcBA-IGS nucleotide sequence analysis.
作者信息
Dyble Julianne, Paerl Hans W, Neilan Brett A
机构信息
Institute of Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, North Carolina 28557, USA.
出版信息
Appl Environ Microbiol. 2002 May;68(5):2567-71. doi: 10.1128/AEM.68.5.2567-2571.2002.
Abstract
Isolates of the toxic, N(2)-fixing species Cylindrospermopsis raciborskii from various geographic locations were analyzed with respect to their genetic diversity based on the nifH and cpcBA-IGS genes. Gene sequences clustered according to their geographic origin, with the nifH sequences separating into European, Australian, and American groups and the cpcBA-IGS sequences separating into American and European or Australian groups. PCR primers for both genes were designed to exclusively amplify DNA from Cylindrospermopsis species, and an additional primer set for cpcBA-IGS was designed to specifically amplify the American C. raciborskii strains.
摘要
基于nifH和cpcBA-IGS基因，对来自不同地理位置的有毒固氮物种柱孢藻（Cylindrospermopsis raciborskii）的分离株进行了遗传多样性分析。基因序列根据其地理来源聚类，nifH序列分为欧洲、澳大利亚和美洲组，cpcBA-IGS序列分为美洲和欧洲或澳大利亚组。这两个基因的PCR引物设计为专门扩增柱孢藻属物种的DNA，另外还设计了一组cpcBA-IGS引物，专门扩增美洲柱孢藻菌株的DNA。
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