Department of Neurosurgery, University Clinical Centre Maribor, Ljubljanska 5, SI-2000 Maribor, Slovenia.
Anticancer Res. 2010 Dec;30(12):4851-60.
To study the neuropathology and selected tumour markers of malignant gliomas, an animal glioma model was developed using the implantation of U87 human glioblastoma cells into chick chorioallantoic membrane. The immunohistochemical characteristics were studied and compared with an orthotopic rodent model.
The U87 cell suspension was inoculated onto the chick chorioallantoic membrane on embryonic day seven and into the brain of nude rats. Brain tumour sections were examined for various known tumour markers by routine haematoxylin and eosin staining and immunohistochemical analyses.
The immunohistochemical analyses showed that S100 protein, glial fibrillary acidic protein and synaptophysin expressions, initially present in tissue culture, were lost in both models. Persistent kallikrein, CD68 and vimentin expressions in U87 cells, as well as in both animal tumour models, were detected. The percentage of p53-positive nuclei, which was higher in the tumours grown on the chick chorioallantoic membrane than in rats, did not correlate with the Ki-67 labelling index. Strong cathepsin expression was maintained from the cell culture to both tumour models. CD3-positive cells and numerous leukocytes, but no CD20-positive cells were detected in any of the animal samples, indicating the immunological response of the host to be primarily cellular. Stronger immune reaction for vascular endothelial growth factor in rats correlated with an observed increase in vascular proliferation in these tumours.
A simple, fast-growing, cheap and well-defined chick chorioallantoic membrane model of glioma was established, providing a basis for further experimental studies of genetic and protein expression during human glioma tumourigenesis. This model may possibly replace some rodent models for selective studies.
为了研究恶性神经胶质瘤的神经病理学和特定肿瘤标志物，我们采用将 U87 人胶质母细胞瘤细胞植入鸡胚绒毛尿囊膜的方法，建立了一个动物神经胶质瘤模型。我们对其免疫组织化学特征进行了研究，并与原位啮齿动物模型进行了比较。
将 U87 细胞悬液接种到鸡胚绒毛尿囊膜上（胚胎第 7 天），或接种到裸鼠脑内。通过常规苏木精和伊红染色及免疫组织化学分析，对脑肿瘤切片进行了各种已知肿瘤标志物的检测。
免疫组织化学分析显示，在组织培养中最初存在的 S100 蛋白、胶质纤维酸性蛋白和突触素表达，在两种模型中均消失。在 U87 细胞和两种动物肿瘤模型中均检测到持续表达的激肽释放酶、CD68 和波形蛋白。在鸡胚绒毛尿囊膜上生长的肿瘤中，p53 阳性核的比例高于大鼠，但其与 Ki-67 标记指数无相关性。在细胞培养和两种肿瘤模型中，均保持了组织蛋白酶的强表达。在任何动物样本中，均检测到 CD3 阳性细胞和大量白细胞，但未检测到 CD20 阳性细胞，表明宿主的免疫反应主要是细胞性的。在大鼠中，血管内皮生长因子的强烈免疫反应与这些肿瘤中血管增殖的观察增加相关。
建立了一种简单、快速生长、廉价且明确的鸡胚绒毛尿囊膜神经胶质瘤模型，为进一步研究人类神经胶质瘤发生过程中的基因和蛋白质表达提供了基础。该模型可能会替代某些啮齿动物模型，用于选择性研究。
