Cooksey Robert C, Abbadi Said H, Woodley Charles L, Sikes David, Wasfy Momtaz, Crawford Jack T, Mahoney Frank
Division of AIDS, STD, and TB Laboratory Research, Centers for Disease Control and Prevention, Mail Stop F-08, Atlanta, GA 30333, USA.
J Clin Microbiol. 2002 May;40(5):1651-5. doi: 10.1128/JCM.40.5.1651-1655.2002.
Mycobacterium tuberculosis complex isolates from cerebrospinal fluid of 67 meningitis patients were obtained from six fever hospitals in Egypt. One M. bovis and 66 M. tuberculosis isolates were identified by PCR-restriction fragment length polymorphism (RFLP) analysis of oxyR. Among the M. tuberculosis isolates, 53 unique strain types (with 3 to 16 copies of IS6110) were found by RFLP analyses. Nine clusters (eight with two isolates each and one with six isolates) were also found. Thirty-six spoligotypes, including at least 10 that have been previously reported from other countries, were also observed. Forty-one (62.1%) of the isolates were in spoligotype clusters, and 22 (33%) of the isolates were in RFLP clusters. Fifty-one of the isolates were susceptible in vitro to all of the antituberculosis drugs tested, 11 were monoresistant to capreomycin, rifampin, isoniazid (INH), pyrazinamide, or streptomycin (STR), 4 were resistant to STR and INH, and 1 was resistant to STR, INH, and ethambutol.
从埃及六家发热医院的67例脑膜炎患者的脑脊液中分离出结核分枝杆菌复合群菌株。通过对oxyR进行聚合酶链反应-限制性片段长度多态性（RFLP）分析，鉴定出1株牛分枝杆菌和66株结核分枝杆菌。在结核分枝杆菌分离株中，通过RFLP分析发现了53种独特的菌株类型（IS6110有3至16个拷贝）。还发现了9个簇（8个簇各有2株，1个簇有6株）。还观察到36种间隔寡核苷酸分型，其中至少10种先前已在其他国家报道过。41株（62.1%）分离株属于间隔寡核苷酸分型簇，22株（33%）分离株属于RFLP簇。51株分离株在体外对所有测试的抗结核药物敏感，11株对卷曲霉素、利福平、异烟肼（INH）、吡嗪酰胺或链霉素（STR）单药耐药，4株对STR和INH耐药，1株对STR、INH和乙胺丁醇耐药。
