Alfa Cisse O, Ouattara A, Thellier M, Accoceberry I, Biligui S, Minta D, Doumbo O, Desportes-Livage I, Thera M A, Danis M, Datry A
Unité INSERM 511, Laboratoire de Parasitologie-Mycologie, Centre Hospitalier-Universitaire de la Pitié-Salpêtrière, 47 Boulevard de l'Hôpital, 75013 Paris, France.
J Clin Microbiol. 2002 May;40(5):1715-8. doi: 10.1128/JCM.40.5.1715-1718.2002.
A 2-month study was carried out in Mali to evaluate an immunofluorescent-antibody test (IFAT) using monoclonal probes specific for Enterocytozoon bieneusi or Encephalitozoon intestinalis. Sixty-one human immunodeficiency virus (HIV)-seropositive adult patients and 71 immunocompetent children were enrolled. Microsporidia were detected in stools from 8 of 61 patients (13.1%) seropositive for HIV. A single species, E. bieneusi, was identified. All the children were negative for microsporidia. The sensitivity and specificity of IFAT were 100% compared with those of PCR, which was used as the "gold standard." Moreover, species identification by IFAT was more rapid and less expensive than that by PCR. These results show the suitability of IFAT for detection of microsporidia in developing countries.
在马里进行了一项为期2个月的研究，以评估一种使用针对比氏肠微孢子虫或肠道脑炎微孢子虫的单克隆探针的免疫荧光抗体试验（IFAT）。招募了61名成人人类免疫缺陷病毒（HIV）血清阳性患者和71名免疫功能正常的儿童。在61名HIV血清阳性患者中的8名（13.1%）的粪便中检测到微孢子虫。鉴定出单一物种比氏肠微孢子虫。所有儿童的微孢子虫检测均为阴性。与被用作“金标准”的聚合酶链反应（PCR）相比，IFAT的敏感性和特异性均为100%。此外，与PCR相比，IFAT进行物种鉴定更快且成本更低。这些结果表明IFAT适用于在发展中国家检测微孢子虫。
