Suzuki Takashi, Nakagawa Masahiro, Yoshikawa Ayumu, Sasagawa Noboru, Yoshimori Tamotsu, Ohsumi Yoshinori, Nishino Ichizo, Ishiura Shoichi, Nonaka Ikuya
Department of Life Science, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902.
J Biochem. 2002 May;131(5):647-51. doi: 10.1093/oxfordjournals.jbchem.a003147.
Chloroquine myopathy is a drug poisoning disease involving rimmed vacuole formation. By Western blot analysis, we investigated posttranslational modification of LC3 in cultured cells with a high concentration of chloroquine, and found that the autophagosome membrane-bound form of LC3 increased dose-dependently. We also constructed a disease model by excessive chloroquine injection into rats and unusual immunohistochemical alteration was chased using anti-LC3 antibodies. With chloroquine treatment, muscle atrophy occurred predominantly in soleus muscle and unusual autophagosomes were accumulated. Therefore, we concluded that autophagy plays an important role in rimmed vacuole formation in certain muscular atrophies.
