Zimmerman A M, Zimmerman S, Thomas J, Ginzburg I
FEBS Lett. 1983 Dec 12;164(2):318-21. doi: 10.1016/0014-5793(83)80309-3.
Poly(A)-containing mRNA was isolated from division synchronized populations of the ciliated protozoan, Tetrahymena pyriformis. The level of tubulin and actin mRNA at specific cell cycle stages was analyzed by hybridization to tubulin and actin cDNA probes and by gel analysis of their in vitro translation products. The pattern of fluctuation of tubulin mRNA levels was similar to that observed for the in vivo tubulin synthesis previously reported [1]. This suggests that as the cells progress through the cell cycle, tubulin synthesis is controlled at the mRNA level. There was little fluctuation of actin synthesis or actin mRNA levels during the cell cycle, which may be indicative of a different regulatory mechanism for actin than for tubulin.
从纤毛原生动物梨形四膜虫的分裂同步群体中分离出含多聚腺苷酸（Poly(A)）的信使核糖核酸（mRNA）。通过与微管蛋白和肌动蛋白互补脱氧核糖核酸（cDNA）探针杂交以及对其体外翻译产物进行凝胶分析，分析了特定细胞周期阶段微管蛋白和肌动蛋白mRNA的水平。微管蛋白mRNA水平的波动模式与先前报道的体内微管蛋白合成的波动模式相似[1]。这表明，随着细胞在细胞周期中进展，微管蛋白合成在mRNA水平受到控制。在细胞周期中，肌动蛋白合成或肌动蛋白mRNA水平几乎没有波动，这可能表明肌动蛋白的调节机制与微管蛋白不同。
