Su Chang-Chao, Cheng Xia-Nian, Zhai Hu-Qu, Wan Jian-Min
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Rice Research Institute, Nanjing Agricultural University, Nanjing 210095, China.
Yi Chuan Xue Bao. 2002 Apr;29(4):332-8.
A mapping population of 98 BC1F9 lines (backcross inbred lines: BILs), derived from a backcross of Nipponbare (japonica)/Kasalath (indica)//Nipponbare by the single-seed descent methods, was used to detect quantitative trait loci (QTL) for resistance to brown planthopper (BPH), Nilaparvata lugens (Stål). Seedbox Screening Technique (SST) was applied to evaluate the reactions of two parents and 98 BILs to BPH at the seedling stage, and the entries were graded on ratio of dead seedlings. A total of three QTL controlling BPH resistance were detected on chromosomes 2, 10 and 12, respectively. Individual QTL accounted for between 10.4% and 16.6% of the phenotypic variance, and the resistance of all the three QTL came from Kasalath, the moderate resistance parent. These QTL should be useful in breeding of varieties resistant to BPH in marker-assisted selection (MAS) program.
通过单粒传法，以日本晴（粳稻）/卡萨拉思（籼稻）//日本晴回交构建了一个由98个BC1F9株系（回交自交系：BILs）组成的作图群体，用于检测对褐飞虱（Nilaparvata lugens (Stål)）抗性的数量性状位点（QTL）。采用秧田筛选技术（SST）在苗期评估两个亲本和98个BILs对褐飞虱的反应，并根据死苗率对各材料进行分级。在第2、10和12号染色体上分别检测到总共3个控制褐飞虱抗性的QTL。单个QTL解释的表型变异在10.4%至16.6%之间，所有这3个QTL的抗性均来自中度抗性亲本卡萨拉思。这些QTL在标记辅助选择（MAS）计划中对培育抗褐飞虱品种应具有重要作用。
