Liang Huizhen, Yu Yongliang, Yang Hongqi, Xu Lanjie, Dong Wei, Du Hua, Cui Weiwen, Zhang Haiyang
Henan Sesame Research Center, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China,
Theor Appl Genet. 2014 Oct;127(10):2127-37. doi: 10.1007/s00122-014-2366-z. Epub 2014 Aug 22.
This study provides a foundation for further research on root genetic regulation and molecular breeding with emphasis on correlations among root traits to ensure robust root growth and well-developed root systems. A set of 447 recombinant inbred lines (RILs) derived from a cross between Jingdou23 (cultivar, female parent) and ZDD2315 (semi-wild, male parent) were used to analyze inheritance and detect QTLs related to root traits at the seedling stage using major gene plus polygene mixed inheritance analysis and composite interval mapping. The results showed that maximum root length (MRL) was controlled by three equivalent major genes, lateral root number (LRN) was controlled by two overlapping major genes, root weight (RW) and volume (RV) were controlled by four equivalent major genes. Hypocotyl length (HL) was controlled by four additive main genes, and hypocotyl weight (HW) was controlled by four additive and additive × additive epistatic, major genes; however, polygene effects were not detected in these traits. Shoot weight (SW) was controlled by multi-gene effects, but major gene effects were not detected. Twenty-four QTLs for MRL, LRN, RW, RV, SW, HL, HW were mapped on LG A1 (chromosome 5), LG A2 (chromosome 8), LG B1 (chromosome 11), LG B2 (chromosome 14), LG C2 (chromosome 6), LG D1b (chromosome 2), LG F_1 (chromosome 13), LG G (chromosome 18), LG H_1 (chromosome 12), LG H_2 (chromosome 12), LG I (chromosome 20), LG K_2 (chromosome 9), LG L (chromosome 19), LG M (chromosome 7), LG N (chromosome 3), LG O (chromosome 10), separately. Root traits were shown to have complex genetic mechanisms at the seedling stage, SW was controlled by multi-gene effects, and the other six traits were controlled by major gene effects. It is concluded that correlations among root traits must be considered to improve the development of beneficial root traits.
本研究为进一步开展根系遗传调控和分子育种研究奠定了基础，重点在于根系性状之间的相关性，以确保根系健壮生长和根系系统发育良好。利用由京豆23（品种，母本）和ZDD2315（半野生，父本）杂交产生的一套447个重组自交系（RILs），采用主基因加多基因混合遗传分析和复合区间作图法，分析苗期根系性状的遗传并检测相关QTL。结果表明，最大根长（MRL）受3个等效主基因控制，侧根数（LRN）受2个重叠主基因控制，根重（RW）和根体积（RV）受4个等效主基因控制。下胚轴长度（HL）受4个加性主基因控制，下胚轴重量（HW）受4个加性和加性×加性上位性主基因控制；然而，在这些性状中未检测到多基因效应。地上部重量（SW）受多基因效应控制，但未检测到主基因效应。将24个与MRL、LRN、RW、RV、SW、HL、HW相关的QTL分别定位在LG A1（第5染色体）、LG A2（第8染色体）、LG B1（第11染色体）、LG B2（第14染色体）、LG C2（第6染色体）、LG D1b（第2染色体）、LG F_1（第13染色体）、LG G（第18染色体）、LG H_1（第12染色体）、LG H_2（第12染色体）、LG I（第20染色体）、LG K_2（第9染色体）、LG L（第19染色体）、LG M（第7染色体）、LG N（第3染色体）、LG O（第10染色体）上。根系性状在苗期表现出复杂的遗传机制，SW受多基因效应控制，其他6个性状受主基因效应控制。研究得出结论，为改善有益根系性状的发育，必须考虑根系性状之间的相关性。
