Sun Li-Hong, Wang Chun-Ming, Su Chang-Chao, Liu Yu-Qiang, Zhai Hu-Qu, Wan Jian-Min
National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, China.
Yi Chuan Xue Bao. 2006 Aug;33(8):717-23. doi: 10.1016/S0379-4172(06)60104-2.
Nilaparvata lugens Stål (brown planthopper, BPH), is one of the major insect pests of rice (Oryza sativa L.) in the temperate rice-growing region. In this study, ASD7 harboring a BPH resistance gene bph2 was crossed to a susceptible cultivar C418, a japonica restorer line. BPH resistance was evaluated using 134 F2:3 lines derived from the cross between "ASD7" and "C418". SSR assay and linkage analysis were carried out to detect bph2. As a result, the resistant gene bph2 in ASD7 was successfully mapped between RM7102 and RM463 on the long arm of chromosome 12, with distances of 7.6 cM and 7.2 cM, respectively. Meanwhile, both phenotypic selection and marker-assisted selection (MAS) were conducted in the BC1F1 and BC2F1 populations. Selection efficiencies of RM7102 and RM463 were determined to be 89.9% and 91.2%, respectively. It would be very beneficial for BPH resistance improvement by using MAS of this gene.
褐飞虱（Nilaparvata lugens Stål）是温带水稻种植区水稻（Oryza sativa L.）的主要害虫之一。在本研究中，携带褐飞虱抗性基因bph2的ASD7与感病品种C418（一个粳稻恢复系）进行杂交。利用“ASD7”和“C418”杂交产生的134个F2:3株系评估褐飞虱抗性。进行SSR分析和连锁分析以检测bph2。结果表明，ASD7中的抗性基因bph2成功定位在第12号染色体长臂上的RM7102和RM463之间，距离分别为7.6 cM和7.2 cM。同时，在BC1F1和BC2F1群体中进行了表型选择和标记辅助选择（MAS）。确定RM7102和RM463的选择效率分别为89.9%和91.2%。利用该基因的MAS对提高褐飞虱抗性将非常有益。
