Cranny A, Crowley P, Whelan A
Central Pathology Laboratory, St James's Hospital, Dublin, Ireland.
Clin Exp Immunol. 2002 May;128(2):275-8. doi: 10.1046/j.1365-2249.2002.01820.x.
The effect of human placental lactogen (hPL), a member of the somatomammotrophin family, on the regulation of the scavenger receptor molecules CD14 and CD163 on human monocytes cultured for 48h was investigated. Cells were cultured in the presence or absence of the hormone and also in the presence or absence of IFN-gamma and dexamethasone. Monocytes cultured in the presence of hPL showed a significant increase in the expression of CD14 in both males and females compared to background. When IFN-gamma and dexamethasone were added to the cultures, CD14 expression was decreased and was not rescued by the presence of hPL. hPL alone had no effect on the expression of CD163 on cultured monocytes from either gender, although cells cultured in the presence of IFN-gamma and dexamethasone showed a profound increase in their expression of CD163. This expression was augmented further by the presence of hPL in the cultures over a 48-h period. These results support the hypothesis of a potential role of this hormone in the regulation of the innate immune response.
研究了人胎盘催乳素（hPL）（生长催乳素家族的一员）对培养48小时的人单核细胞上清道夫受体分子CD14和CD163调节的影响。细胞在有或无该激素的情况下培养，同时也在有或无干扰素-γ和地塞米松的情况下培养。与空白对照相比，在hPL存在下培养的单核细胞中，男性和女性的CD14表达均显著增加。当向培养物中添加干扰素-γ和地塞米松时，CD14表达降低，且hPL的存在无法使其恢复。单独的hPL对两种性别人单核细胞培养物中CD163的表达均无影响，尽管在干扰素-γ和地塞米松存在下培养的细胞其CD163表达显著增加。在48小时的培养期间，培养物中hPL的存在进一步增强了这种表达。这些结果支持了该激素在调节先天性免疫反应中可能发挥作用的假说。
