用于检测牛粪便样本中轮状病毒的侧向流动免疫分析（免疫层析）的开发、评估及应用
Development, evaluation, and application of lateral-flow immunoassay (immunochromatography) for detection of rotavirus in bovine fecal samples.
作者信息
Al-Yousif Yousif, Anderson Joe, Chard-Bergstrom Cindy, Kapil Sanjay
机构信息
Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506, USA.
出版信息
Clin Diagn Lab Immunol. 2002 May;9(3):723-5. doi: 10.1128/cdli.9.3.723-724.2002.
Abstract
A lateral-flow immunoassay (LFT) was developed to detect bovine rotavirus in fecal samples. Using samples (n = 74) from diarrheic calves, a comparison of the LFT with a commercial latex agglutination test (LAT) and transmission electron microscopy (EM) was conducted. When EM was used as the reference method, initial studies of 29 samples indicated 70 and 80% sensitivities of the LFT and LAT, respectively, with both being 100% specific. When the LAT was the reference test, the LFT was 75% sensitive and 91% specific. Additional specimens (n = 45) were tested by the LFT and LAT alone, and results were identical for both methods.
摘要
开发了一种侧向流动免疫测定法（LFT）来检测粪便样本中的牛轮状病毒。使用腹泻小牛的样本（n = 74），对LFT与商业乳胶凝集试验（LAT）和透射电子显微镜（EM）进行了比较。当以EM作为参考方法时，对29个样本的初步研究表明，LFT和LAT的敏感性分别为70%和80%，两者的特异性均为100%。当以LAT作为参考试验时，LFT的敏感性为75%，特异性为91%。仅通过LFT和LAT对另外45个样本进行了检测，两种方法的结果相同。
相似文献
1
Development, evaluation, and application of lateral-flow immunoassay (immunochromatography) for detection of rotavirus in bovine fecal samples.用于检测牛粪便样本中轮状病毒的侧向流动免疫分析（免疫层析）的开发、评估及应用
Clin Diagn Lab Immunol. 2002 May;9(3):723-5. doi: 10.1128/cdli.9.3.723-724.2002.
2
Detection of group- and subgroup-specific antigens of bovine rotaviruses in Bangladesh.孟加拉国牛轮状病毒群特异性和亚群特异性抗原的检测
J Diarrhoeal Dis Res. 1999 Jun;17(2):81-4.
3
Comparison of electron microscopy, enzyme-linked immunosorbent assay and latex agglutination for the detection of bovine rotavirus in faeces.电子显微镜检查、酶联免疫吸附测定和乳胶凝集试验检测粪便中牛轮状病毒的比较
J S Afr Vet Assoc. 1997 Sep;68(3):93-6. doi: 10.4102/jsava.v68i3.883.
4
Evaluation of a latex agglutination kit (Virogen Rotatest) for detection of bovine rotavirus in fecal samples.评估一种用于检测粪便样本中牛轮状病毒的乳胶凝集试剂盒（Virogen Rotatest）。
Clin Diagn Lab Immunol. 2001 May;8(3):496-8. doi: 10.1128/CDLI.8.3.496-498.2001.
5
Comparison of electron microscopy, ELISA, real time RT-PCR and insulated isothermal RT-PCR for the detection of Rotavirus group A (RVA) in feces of different animal species.电子显微镜检查、酶联免疫吸附测定、实时逆转录聚合酶链反应和绝缘等温逆转录聚合酶链反应用于检测不同动物物种粪便中A组轮状病毒（RVA）的比较
J Virol Methods. 2016 Sep;235:99-104. doi: 10.1016/j.jviromet.2016.05.006. Epub 2016 May 11.
6
[Rotavirus infection of the calf. 2. Use of enzyme immunoassay in the detection of virus antigen in fecal samples].[犊牛轮状病毒感染。2. 酶免疫测定法在粪便样本病毒抗原检测中的应用]
Arch Exp Veterinarmed. 1987 Nov;41(6):939-43.
7
Evaluation of the second generation of a commercial latex agglutination test for the detection of rotavirus antigens in fecal samples.评价第二代商用胶乳凝集试验检测粪便样本中轮状病毒抗原。
J Clin Virol. 2013 May;57(1):88-90. doi: 10.1016/j.jcv.2013.01.011. Epub 2013 Feb 9.
8
9
Use of polymerase chain reaction for the differentiation of Group A bovine rotavirus G6, G8, and G10 genotypes in the North Island of New Zealand.利用聚合酶链反应鉴别新西兰北岛A组牛轮状病毒G6、G8和G10基因型
N Z Vet J. 2008 Oct;56(5):218-21. doi: 10.1080/00480169.2008.36836.
10
Rapid and sensitive detection of bovine coronavirus and group a bovine rotavirus from fecal samples by using one-step duplex RT-PCR assay.通过一步法双重逆转录聚合酶链反应（RT-PCR）检测法从粪便样本中快速灵敏地检测牛冠状病毒和A组牛轮状病毒。
J Vet Med Sci. 2011 Apr;73(4):531-4. doi: 10.1292/jvms.10-0401. Epub 2010 Dec 6.
引用本文的文献
1
Development and clinical evaluation of a rapid antibody lateral flow assay for the diagnosis of SARS-CoV-2 infection.一种用于 SARS-CoV-2 感染诊断的快速抗体侧向流动检测试剂的研发和临床评估。
BMC Infect Dis. 2021 Aug 23;21(1):860. doi: 10.1186/s12879-021-06568-9.
2
2D materials as a diagnostic platform for the detection and sensing of the SARS-CoV-2 virus: a bird's-eye view.二维材料作为 SARS-CoV-2 病毒检测和传感的诊断平台：鸟瞰图。
J Mater Chem B. 2021 Jun 16;9(23):4608-4619. doi: 10.1039/d1tb00071c.
3
Assessing the Immunochromatographic Test Strip for Serological Detection of Bovine Babesiosis in Uganda.乌干达用于牛巴贝斯虫病血清学检测的免疫层析试纸条评估
Microorganisms. 2020 Jul 24;8(8):1110. doi: 10.3390/microorganisms8081110.
4
A quantum dot-based lateral flow immunoassay for the rapid, quantitative, and sensitive detection of specific IgE for mite allergens in sera from patients with allergic rhinitis.基于量子点的侧向流动免疫分析用于快速、定量和灵敏地检测过敏性鼻炎患者血清中特异性 IgE 螨过敏原。
Anal Bioanal Chem. 2020 Mar;412(8):1785-1794. doi: 10.1007/s00216-020-02422-0. Epub 2020 Feb 12.
5
Rapid and sensitive detection of Yersinia pestis by lateral-flow assay in simulated clinical samples.基于侧流层析法的模拟临床样本中鼠疫耶尔森氏菌的快速灵敏检测。
BMC Infect Dis. 2018 Aug 14;18(1):402. doi: 10.1186/s12879-018-3315-2.
6
Immunodiagnosis of Fasciola gigantica Infection Using Monoclonal Antibody-Based Sandwich ELISA and Immunochromatographic Assay for Detection of Circulating Cathepsin L1 Protease.使用基于单克隆抗体的夹心酶联免疫吸附测定法和免疫层析法检测循环组织蛋白酶L1蛋白酶对巨片形吸虫感染进行免疫诊断
PLoS One. 2016 Jan 5;11(1):e0145650. doi: 10.1371/journal.pone.0145650. eCollection 2016.
7
Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Bovine Rotavirus.反转录环介导等温扩增检测试剂盒快速检测牛轮状病毒
BMC Vet Res. 2012 Aug 15;8:133. doi: 10.1186/1746-6148-8-133.
8
Comparison of three diagnostic techniques for detection of rotavirus and coronavirus in calf faeces in Australia.澳大利亚犊牛粪便中轮状病毒和冠状病毒检测的三种诊断技术比较
Aust Vet J. 2012 Apr;90(4):122-9. doi: 10.1111/j.1751-0813.2011.00891.x.
9
Development and application of one-step multiplex reverse transcription PCR for simultaneous detection of five diarrheal viruses in adult cattle.一步法多重逆转录聚合酶链反应同时检测成年牛五种腹泻病毒的开发与应用。
Arch Virol. 2012 Jun;157(6):1063-9. doi: 10.1007/s00705-012-1271-5. Epub 2012 Mar 11.
10
A Brief Review on Diagnosis of Foot-and-Mouth Disease of Livestock: Conventional to Molecular Tools.家畜口蹄疫诊断简述：从传统方法到分子工具
Vet Med Int. 2011;2011:905768. doi: 10.4061/2011/905768. Epub 2011 Jul 6.
本文引用的文献
1
Evaluation of a latex agglutination kit (Virogen Rotatest) for detection of bovine rotavirus in fecal samples.评估一种用于检测粪便样本中牛轮状病毒的乳胶凝集试剂盒（Virogen Rotatest）。
Clin Diagn Lab Immunol. 2001 May;8(3):496-8. doi: 10.1128/CDLI.8.3.496-498.2001.
2
Comparison of electron microscopy, enzyme-linked immunosorbent assay and latex agglutination for the detection of bovine rotavirus in faeces.电子显微镜检查、酶联免疫吸附测定和乳胶凝集试验检测粪便中牛轮状病毒的比较
J S Afr Vet Assoc. 1997 Sep;68(3):93-6. doi: 10.4102/jsava.v68i3.883.
3
4
Epidemiology of rotavirus infection in broiler chickens: recognition of four serogroups.肉鸡轮状病毒感染的流行病学：四种血清群的识别
Arch Virol. 1984;81(1-2):113-21. doi: 10.1007/BF01309301.
5
Comparison of atypical rotaviruses from calves, piglets, lambs and man.来自犊牛、仔猪、羔羊和人类的非典型轮状病毒的比较。
J Gen Virol. 1984 May;65 ( Pt 5):909-14. doi: 10.1099/0022-1317-65-5-909.
6
7
Rotavirus: the major etiologic agent of severe infantile diarrhea may be controllable by a "Jennerian" approach to vaccination.轮状病毒：严重婴幼儿腹泻的主要病原体或许可通过“詹纳式”疫苗接种方法加以控制。
J Infect Dis. 1986 May;153(5):815-22. doi: 10.1093/infdis/153.5.815.
Zotero 插件