用于检测牛粪便样本中轮状病毒的侧向流动免疫分析（免疫层析）的开发、评估及应用
Development, evaluation, and application of lateral-flow immunoassay (immunochromatography) for detection of rotavirus in bovine fecal samples.
作者信息
Al-Yousif Yousif, Anderson Joe, Chard-Bergstrom Cindy, Kapil Sanjay
机构信息
Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506, USA.
出版信息
Clin Diagn Lab Immunol. 2002 May;9(3):723-5. doi: 10.1128/cdli.9.3.723-724.2002.
Abstract
A lateral-flow immunoassay (LFT) was developed to detect bovine rotavirus in fecal samples. Using samples (n = 74) from diarrheic calves, a comparison of the LFT with a commercial latex agglutination test (LAT) and transmission electron microscopy (EM) was conducted. When EM was used as the reference method, initial studies of 29 samples indicated 70 and 80% sensitivities of the LFT and LAT, respectively, with both being 100% specific. When the LAT was the reference test, the LFT was 75% sensitive and 91% specific. Additional specimens (n = 45) were tested by the LFT and LAT alone, and results were identical for both methods.
摘要
开发了一种侧向流动免疫测定法（LFT）来检测粪便样本中的牛轮状病毒。使用腹泻小牛的样本（n = 74），对LFT与商业乳胶凝集试验（LAT）和透射电子显微镜（EM）进行了比较。当以EM作为参考方法时，对29个样本的初步研究表明，LFT和LAT的敏感性分别为70%和80%，两者的特异性均为100%。当以LAT作为参考试验时，LFT的敏感性为75%，特异性为91%。仅通过LFT和LAT对另外45个样本进行了检测，两种方法的结果相同。
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