Department of Infectomics and Molecular Pathogenesis, CINVESTAV-IPN. Av. IPN 2508, San Pedro Zacatenco, C.P. 07360, México, D.F., Mexico.
J Clin Virol. 2013 May;57(1):88-90. doi: 10.1016/j.jcv.2013.01.011. Epub 2013 Feb 9.
Despite vaccine availability, the infection rate and disease burden associated with rotavirus infection are still high. Thus, accurate diagnosis of rotavirus infection continues to be necessary for proper patient clinical management and disease control.
To evaluate the performance of a novel, second generation, commercial latex tests (Pastorex™ Rotavirus latex agglutination test, BIORAD, Marnes-La-Coquette, France), for the detection of rotavirus in human feces.
Using 166 fecal samples collected from children with acute diarrhea, and previously tested for rotavirus, calicivirus and astrovirus, the second generation Pastorex™ Rotavirus latex agglutination test was evaluated.
The test showed a sensitivity of 85.9% and a specificity of 97.7%. Positive and negative predicted values for the test were 97% and 88%, respectively. The results suggest that this commercial test is a good alternative for rotavirus diagnosis.
尽管有疫苗可用，但轮状病毒感染的感染率和疾病负担仍然很高。因此，准确诊断轮状病毒感染对于患者的临床管理和疾病控制仍然是必要的。
评估一种新型第二代商业乳胶检测（Pastorex ™轮状病毒乳胶凝集检测，BIORAD，Marnes-La-Coquette，法国）在检测人粪便中轮状病毒方面的性能。
使用 166 份从患有急性腹泻的儿童中收集的粪便样本，这些样本之前已经检测过轮状病毒、杯状病毒和星状病毒，对第二代 Pastorex ™轮状病毒乳胶凝集检测进行了评估。
该检测的敏感性为 85.9%，特异性为 97.7%。该检测的阳性和阴性预测值分别为 97%和 88%。结果表明，该商业检测是轮状病毒诊断的一种较好的替代方法。
