[EBV BARF1基因与佛波酯诱导猴肾上皮细胞恶性转化]

[Malignant transformation of monkey kidney epithelial cell induced by EBV BARF1 gene and TPA].

作者信息

Guo X, Sheng W, Zhang Y

机构信息

Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2001 Dec;15(4):321-3.

PMID:11986715

Abstract

BACKGROUND

To study the effect of Epstein-Barr virus (EBV) BARF1 gene alone and synergistic effect of the gene and TPA in carcinogenesis.

METHODS

PT1 and PT7 cell line which were immortalized monkey kidney epithelial cells induced by the BARF1 gene of EB virus.PT1 and PT7 cell were cultured with 5 ng/ml TPA for two weeks. Then the nude mice and scid mice were injected with PT1, PT7, PT1+TPA, PT7+TPA cells, respectively.

RESULTS

The immortalized PT1 and PT7 cells could not develop the tumor in nude mice, but could develop the tumor in scid mice (severe combined immune deficiency mice). It took about 45-60 days for development of tumor. After adding the tumor promoter TPA, the PT1 and PT7 cells developed the tumor either in nude mice or scid mice. It needed about 20-22 days in nude mice and 5-7 days in scid mice for development of tumor. PCR amplification revealed EBV-BARF1 gene in tumor tissues.

CONCLUSIONS

EB virus BARF1 gene is another oncogene. Malignant transformation of the primary monkey kidney epithelial cell can be induced with BARF1 gene,even without tumor promoter.

摘要

背景

研究爱泼斯坦-巴尔病毒（EBV）BARF1基因单独作用以及该基因与佛波酯（TPA）协同作用在致癌过程中的影响。

方法

PT1和PT7细胞系是由EB病毒的BARF1基因诱导永生化的猴肾上皮细胞。将PT1和PT7细胞用5 ng/ml TPA培养两周。然后分别将PT1、PT7、PT1 + TPA、PT7 + TPA细胞注射到裸鼠和重症联合免疫缺陷（scid）小鼠体内。

结果

永生化的PT1和PT7细胞在裸鼠中不能形成肿瘤，但在scid小鼠（重症联合免疫缺陷小鼠）中能形成肿瘤。肿瘤形成大约需要45 - 60天。加入肿瘤启动子TPA后，PT1和PT7细胞在裸鼠或scid小鼠中均能形成肿瘤。在裸鼠中肿瘤形成需要约20 - 22天，在scid小鼠中需要5 - 7天。PCR扩增显示肿瘤组织中有EBV - BARF1基因。