Saving Kay L, Mankin Peggy E, Gorman Matthew J
Department of Pediatrics, University of Illinois College of Medicine at Peoria, 61637, USA.
J Pediatr Hematol Oncol. 2002 Feb;24(2):120-4. doi: 10.1097/00043426-200202000-00012.
To examine the hypothesis that reticulated platelets and reticulocytes show elevated adhesion receptor expression compared with mature cells in both adult and neonatal cells.
Flow cytometry was used to examine laminin, fibronectin (VLA-6), and thrombospondin (glycoprotein IV [GPIV]) expression in reticulated red cells, reticulated platelets, and older peripherally circulating mature red cells and mature platelets in seven newborn cord blood samples and blood samples from eight adult volunteers.
The difference in the neonatal reticulated platelet percentage of 9.2+/-14.8% was not statistically significant from the adult reticulated platelet percentage of 5.0+/-1.5% in this small population. There was a statistically significant difference between the reticulated cord blood red cell mean of 7.7+/-1.8% and the adult mean of 3.1+/-0.43%. Mean expression of VLA-6 was 96% in adult reticulated platelets, 79% in adult mature platelets, 81% in cord reticulated platelets and 65% in cord mature platelets. Mean expression of GPIV was similar, with corresponding values of 90%, 71%, 78%, and 57%. Reticulated red cells in adults averaged 44% VLA-4 and 46% GPIV; cord reticulocytes were 9% and 15%, respectively.
Reticulated cells newly released from the bone marrow express more adhesive receptors than mature cells in both groups. Cord blood samples showed hypoexpression of both receptor types in red blood cells and platelets.
检验以下假设，即与成体和新生儿细胞中的成熟细胞相比，网织血小板和网织红细胞的黏附受体表达升高。
运用流式细胞术检测七份新生儿脐带血样本以及八名成年志愿者血样中网织红细胞、网织血小板、外周循环中较老的成熟红细胞和成熟血小板中层粘连蛋白、纤连蛋白（VLA - 6）和血小板反应蛋白（糖蛋白IV [GPIV]）的表达。
在这一较小群体中，新生儿网织血小板百分比为9.2±14.8%，与成年网织血小板百分比5.0±1.5%相比，差异无统计学意义。脐带血中网织红细胞均值为7.7±1.8%，与成年人均值3.1±0.43%之间存在统计学显著差异。VLA - 6在成年网织血小板中的平均表达为96%，成年成熟血小板中为79%，脐带网织血小板中为81%，脐带成熟血小板中为65%。GPIV的平均表达情况类似，相应值分别为90%、71%、78%和57%。成年人网织红细胞中VLA - 4平均为44%，GPIV为46%；脐带血网织红细胞中分别为9%和15%。
两组中，从骨髓新释放的网织细胞比成熟细胞表达更多的黏附受体。脐带血样本显示红细胞和血小板中两种受体类型均低表达。
