Joseph M A, Adams D, Maragos J, Saving K L
Department of Pediatrics, University of Illinois, College of Medicine at Peoria 61637, USA.
J Pediatr Hematol Oncol. 1996 Aug;18(3):277-81. doi: 10.1097/00043426-199608000-00008.
Serious disorders of hemostasis occur more often in the small, stressed preterm infant, partly due to immaturity of the newborn hemostatic mechanism. Information regarding the maturational development of platelets in infants has been limited by the large amounts of blood historically needed for platelet studies. The objective of this study was to determine differences between platelets in adults and infants by flow cytometric analysis of "reticulated" platelets.
Eighteen normal adults, 42 healthy term infants, and 27 preterm infants were studied. The infants were subdivided by mode of delivery: vaginal or Caesarean section. Platelet-rich plasma from adult whole blood and infant cord blood samples was divided into aliquots containing 5 X 10(6) platelets, fixed with 1% paraformaldehyde, and stained with thiazole orange for RNA content. The percentage of RNA positive "reticulated" platelets in each aliquot was then determined by flow cytometry.
Group comparisons using ANOVA statistical analysis showed a significant difference (p<0.05) for platelet RNA content between adults and term infants, the adults having a higher percentage of reticulated platelets. Although the difference was smaller, adults also had a higher percentage of reticulated platelets than preterm infants. There was no significant difference in reticulated platelet values between infants within one gestational age group compared by type of delivery.
There are demonstratable differences in adult and infant platelet RNA content that may reflect developmental differences in megakaryocytic/platelet kinetics.
严重的止血障碍在处于应激状态的早产低体重儿中更为常见，部分原因是新生儿止血机制不成熟。以往血小板研究需要大量血液，这限制了有关婴儿血小板成熟发育的信息。本研究的目的是通过对“网织”血小板进行流式细胞术分析，确定成人和婴儿血小板之间的差异。
研究了18名正常成人、42名足月健康婴儿和27名早产婴儿。婴儿按分娩方式细分：阴道分娩或剖宫产。将来自成人全血和婴儿脐带血样本的富含血小板的血浆分成含有5×10⁶个血小板的等分试样，用1%多聚甲醛固定，并用噻唑橙染色以检测RNA含量。然后通过流式细胞术测定每个等分试样中RNA阳性“网织”血小板的百分比。
使用方差分析统计分析进行组间比较显示，成人和足月婴儿的血小板RNA含量存在显著差异（p<0.05），成人的网织血小板百分比更高。虽然差异较小，但成人的网织血小板百分比也高于早产婴儿。按分娩类型比较，同一胎龄组内婴儿的网织血小板值无显著差异。
成人和婴儿的血小板RNA含量存在明显差异，这可能反映了巨核细胞/血小板动力学的发育差异。
