Liu Yueqi, Zou Hanfa, Haginaka Jun
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Hyogo, Japan.
J Sep Sci. 2006 Jul;29(10):1440-6. doi: 10.1002/jssc.200600015.
A novel chiral stationary phase based on chitosan covalently bonded onto silica gels has been prepared and used for the separation of various alpha-amino acid enantiomers as well as alpha-hydroxycarboxylic acid enantiomers by chiral ligand-exchange chromatography with copper(II) as a complexing ion. The methanol content and copper(II) ion concentration in the eluent affected retentivity and enantioselectivity. Furthermore, a plausible chiral recognition mechanism for resolution of alpha-amino acids was proposed.
一种基于壳聚糖共价键合到硅胶上的新型手性固定相已被制备，并用于通过以铜（II）作为络合离子的手性配体交换色谱法分离各种α-氨基酸对映体以及α-羟基羧酸对映体。洗脱液中的甲醇含量和铜（II）离子浓度影响保留率和对映选择性。此外，还提出了一种用于拆分α-氨基酸的合理的手性识别机制。
