在慢性支气管炎急性加重期，嗜酸性粒细胞趋化因子和CCR3表达上调。

Eotaxin and CCR3 are up-regulated in exacerbations of chronic bronchitis.

作者信息

Bocchino V, Bertorelli G, Bertrand C P, Ponath P D, Newman W, Franco C, Marruchella A, Merlini S, Del Donno M, Zhuo X, Olivieri D

机构信息

Department of Respiratory Disease, University of Parma, Italy.

出版信息

Allergy. 2002 Jan;57(1):17-22.

PMID:11991282

Abstract

BACKGROUND

Eosinophils and T lymphocytes represent constant features in the airways of subjects with exacerbated chronic bronchitis. Eotaxin is the most potent and selective eosinophil chemoattractant which can also attracts lymphocytes. The aim of the study was to evaluate the expression of eotaxin and its receptor, CCR3, in bronchial airways during exacerbation of chronic bronchitis.

METHODS

By immunohistochemistry we studied eotaxin and CCR3 expression in the lamina propria of 14 subjects with acute exacerbation of chronic bronchitis. 20 asthmatics, and 8 healthy subjects. We determined the cell types expressing the CCR3 receptor by colocalization experiments. We finally studied the relationship between eotaxin and CCR3 and eosinophils and T lymphocytes.

RESULTS

The number of eotaxin+ and CCR3+ cells was significantly higher in exacerbated chronic bronchitis (P<0.003 and P<0.002) and asthma (P<0.002 and P<0.0001) when compared to healthy subjects. CCR3 was mainly expressed by eosinophils and to a lesser extent by CD4+ and CD8+ lymphocytes. In exacerbated chronic bronchitis the number of CCR3+ cells was strongly correlated to the number of eosinophils (P<0.0002. r=0.85) and to the number of CD4+ lymphocytes (P<0.05, r=0.57).

CONCLUSION

Our study suggests that eotaxin and CCR3 are up-regulated and could be involved in the eosinophil and CD4+ lymphocyte recruitment into the airways which occur during acute exacerbations of chronic bronchitis.

摘要

背景

嗜酸性粒细胞和T淋巴细胞是慢性支气管炎急性加重患者气道中的常见特征。嗜酸性粒细胞趋化因子是最有效且具选择性的嗜酸性粒细胞趋化剂，也可吸引淋巴细胞。本研究旨在评估慢性支气管炎急性加重期支气管气道中嗜酸性粒细胞趋化因子及其受体CCR3的表达情况。

方法

通过免疫组织化学方法，我们研究了14例慢性支气管炎急性加重患者、20例哮喘患者和8例健康受试者固有层中嗜酸性粒细胞趋化因子和CCR3的表达。通过共定位实验确定表达CCR3受体的细胞类型。我们最终研究了嗜酸性粒细胞趋化因子与CCR3以及嗜酸性粒细胞与T淋巴细胞之间的关系。

结果

与健康受试者相比，慢性支气管炎急性加重期（P<0.003和P<0.002）和哮喘（P<0.002和P<0.0001）中嗜酸性粒细胞趋化因子阳性和CCR3阳性细胞数量显著更高。CCR3主要由嗜酸性粒细胞表达，CD4+和CD8+淋巴细胞表达较少。在慢性支气管炎急性加重期，CCR3阳性细胞数量与嗜酸性粒细胞数量（P<0.0002，r=0.85）和CD4+淋巴细胞数量（P<0.05，r=0.57）密切相关。