单纯疱疹病毒1型胸苷激酶突变体的突变谱
Mutation spectra of herpes simplex virus type 1 thymidine kinase mutants.
作者信息
Lu Qiaosheng, Hwang Ying T, Hwang Charles B C
机构信息
Department of Microbiology and Immunology, Medical College, SUNY Upstate Medical University, Syracuse, New York 13210, USA.
出版信息
J Virol. 2002 Jun;76(11):5822-8. doi: 10.1128/jvi.76.11.5822-5828.2002.
Abstract
To examine whether the exonuclease activity intrinsic to the polymerase (Pol) of herpes simplex virus type 1 can influence the mutational spectra, we applied the denaturing gradient gel electrophoresis (DGGE) system combined with sequencing to characterize thymidine kinase mutants isolated from both the wild-type virus and a mutant deficient in exonuclease activity, Y7. Wild-type viruses produced predominantly frameshift mutations (67%), whereas Y7 replicated a significantly lower proportion of frameshifts (21%; P < 0.005). Furthermore, the majority of substitutions were transitional changes in both groups, although they distributed differently. The implications of these findings are discussed.
摘要
为了研究单纯疱疹病毒1型聚合酶（Pol）固有的核酸外切酶活性是否会影响突变谱，我们应用变性梯度凝胶电泳（DGGE）系统结合测序技术，对从野生型病毒和核酸外切酶活性缺陷型突变体Y7中分离出的胸苷激酶突变体进行了表征。野生型病毒主要产生移码突变（67%），而Y7复制的移码突变比例显著较低（21%；P < 0.005）。此外，两组中的大多数替换都是转换变化，尽管它们的分布有所不同。我们讨论了这些发现的意义。
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