Okamoto Yoshiharu, Watanabe Midori, Miyatake Katsuyuki, Morimoto Minoru, Shigemasa Yoshihiro, Minami Saburo
Department of Veterinary Surgery, Faculty of Agriculture, Tottori University, Japan.
Biomaterials. 2002 May;23(9):1975-9. doi: 10.1016/s0142-9612(01)00324-6.
Effects of chitin/chitosan and their oligomers/monomers on migrations of fibroblasts (3T6) and vascular endothelial cells (human umbilical vascular endothelial cell: HUVEC) were evaluated in vitro. In direct migratory assay using the blind well chamber method, migratory activity of 3T6 was seen to be reduced by chitin, chitosan and the chitosan monomer (GlcN). Migratory activity of HUVECs was enhanced by chitin, chitosan and the chitin monomer (GlcNAc), and was reduced by chitosan oligomers and GlcN. Supernatant of 3T6 preincubated with chitin or chitosan reduced migratory activity of 3T6 cells. Supernatant of HUVECs preincubated with chitosan also reduced migratory activity of HUVECs, but supernatant preincubated with chitin had no effect on them. In a proliferation (MTT reduction) assay, none of the samples affected proliferation of either type of cell.
在体外评估了几丁质/壳聚糖及其低聚物/单体对成纤维细胞（3T6）和血管内皮细胞（人脐静脉血管内皮细胞：HUVEC）迁移的影响。在使用盲孔室法的直接迁移试验中，几丁质、壳聚糖和壳聚糖单体（葡糖胺）可降低3T6的迁移活性。几丁质、壳聚糖和几丁质单体（N-乙酰葡糖胺）可增强HUVEC的迁移活性，而壳聚糖低聚物和葡糖胺则可降低其迁移活性。用几丁质或壳聚糖预孵育的3T6的上清液可降低3T6细胞的迁移活性。用壳聚糖预孵育的HUVEC的上清液也可降低HUVEC的迁移活性，但用几丁质预孵育的上清液对其没有影响。在增殖（MTT还原）试验中，所有样品均未影响这两种细胞的增殖。
